Human HHIP (Hedgehog Interacting Protein) CLIA Kit
The Human HHIP (Hedgehog Interacting Protein) CLIA Kit is a highly reliable and sensitive assay designed for the precise measurement of HHIP levels in human samples like serum, plasma, and cell culture supernatants. This kit offers excellent specificity and accuracy, ensuring consistent and reproducible results that are perfect for a wide range of research applications.HHIP is a crucial protein that interacts with the Hedgehog signaling pathway, playing a vital role in regulating cell growth, differentiation, and development.
Dysregulation of the Hedgehog pathway has been implicated in various diseases, including cancer and developmental disorders, making HHIP a valuable biomarker for studying these conditions and potentially developing targeted therapies.With its high sensitivity and specificity, the Human HHIP CLIA Kit is the perfect tool for researchers looking to investigate the role of HHIP in disease pathology and explore new therapeutic strategies. Order your kit today and unlock the potential of HHIP research in your laboratory.
Product Name:
Human HHIP (Hedgehog Interacting Protein) CLIA Kit
SKU:
HUES00532
Target:
Human HHIP (Hedgehog Interacting Protein)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HHIP. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HHIP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HHIP, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HHIP. You can calculate the concentration of Human HHIP in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-101
84-98
89-102
Average (%)
93
91
95
1:4
Range (%)
87-97
96-110
96-106
Average (%)
92
102
101
1:8
Range (%)
85-100
93-107
86-98
Average (%)
92
100
91
1:16
Range (%)
102-117
92-107
101-116
Average (%)
108
99
109
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-116
106
EDTA plasma (n=5)
88-99
94
Cell culture media (n=5)
89-102
94
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.06
625.68
1486.17
197.49
637.73
1348.08
Standard deviation
19.86
74.96
95.26
15.98
49.42
112.7
C V (%)
9.73
11.98
6.41
8.09
7.75
8.36
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HHIP concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HHIP in samples. No significant cross-reactivity or interference between Human HHIP and analogues was observed.
