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Human Hexosaminidase A Alpha (HEXa) ELISA Kit

SKU:
HUDL01327
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06865
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
TSD,Hex-A,Tay Sachs Disease,GM2 Gangliosidosis,Beta-Hexosaminidase Subunit Alpha,Beta-N-Acetylhexosaminidase Subunit Alpha,N-Acetyl-Beta-Glucosaminidase Alpha
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Hexosaminidase A Alpha (HEXa) ELISA Kit

The Human Hexosaminidase A (Alpha HexA) ELISA Kit is a reliable and sensitive tool for detecting levels of hexosaminidase A in human samples such as serum, plasma, and cell culture supernatants. Hexosaminidase A is an important enzyme involved in the breakdown of GM2 gangliosides, with mutations in the HEXA gene leading to Tay-Sachs disease.This ELISA kit is easy to use and provides accurate results, making it suitable for a wide range of research applications.

By measuring hexosaminidase A levels, researchers can gain insights into lysosomal storage disorders, neurodegenerative diseases, and other conditions where hexosaminidase A plays a crucial role.Overall, the Human Hexosaminidase A (Alpha HexA) ELISA Kit is a valuable tool for studying the underlying mechanisms of hexosaminidase A-related diseases and developing potential therapeutic interventions.

Product Name:Human Hexosaminidase A Alpha (HEXa) ELISA Kit
Product Code:HUDL01327
Size:96 Assays
Target:HEXa
Synonyms:TSD, Hex-A, Tay Sachs Disease, GM2 Gangliosidosis, Beta-Hexosaminidase Subunit Alpha, Beta-N-Acetylhexosaminidase Subunit Alpha, N-Acetyl-Beta-Glucosaminidase Alpha
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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