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Human HERC5 Recombinant Protein (RPPB1808)

SKU:
RPPB1808
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UII4
Research Area:
Enzymes
€309
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Description

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Product Name:Human HERC5 Recombinant Protein
Product Code:RPPB1808
Size:20µg
Species:Human
Target:HERC5
Synonyms:HERC5, HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase 5, CEB1, Hect Domain and RLD 5, Cyclin-E-Binding Protein 1, CEBP1, HECT Domain and RCC1-Like Domain-Containing Protein 5, E3 ISG15--Protein Ligase HERC5, Probable E3 Ubiquitin-Protein Ligase HERC5, EC 6.3.2.- , EC 6.3.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:HERC5 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSFDLTVRR NHLIEDVLNQ LSQFENEDLR KELWVSFSGE IGYDLGGVKK EFFYCLFAEM IQPEYGMFMY PEGASCMWFP VKPKFEKKRY FFFGVLCGLS LFNCNVANLP FPLALFKKLL DQMPSLEDLK ELSPDLGKNL QTLLDDEGDN FEEVFYIHFN VHWDRNDTNL IPNGSSITVN QTNKRDYVSK YINYIFNDSV KAVYEEFRRG FYKMCDEDII KLFHPEELKD VIVGNTDYDW KTFEKNARYE PGYNSSHPTI VMFWKAFHKL TLEEKKKFLV FLTGTDRLQM KDLNNMKITF CCPESWNERD PIRALTCFSV LFLPKYSTME TVEEALQEAI NNNRGFG

HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase 5 (HERC5) is a member of the HERC family of ubiquitin ligases, found in a cluster of HERC family genes on chromosome 4. HERC5 is a protein with a HECT domain and 5 RCC1 repeats. The HERC5 protein localizes to the cytoplasm and perinuclear region and serves as an INF-induced E3 protein ligase that mediates ISGylation of protein targets. HERC5 exhibits antiviral activity towards HIV-1, influenza A virus and human papillomavirus. HERC5 is a major E3 ligase for ISG15 conjugation. HERC5 also serves as a positive regulator of innate antiviral response in cells induced by INF. Pro-inflammatory cytokines upregulate HERC5 in endothelial cells. HERC5 is physically connected with polyribosomes, broadly modifies recently synthesized proteins in a cotranslational fashion.

HERC5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 367 amino acids (681-1024 a.a.) and having a molecular mass of 43kDa. HERC5 is fused to a 23 amino acid His-tag at N-terminus.

UniProt Protein Function:HERC5: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. Makes part of the ISGylation machinery that recognizes target proteins in a broad and relatively non-specific manner. Catalyzes ISGylation of IRF3 which results in sustained activation, it attenuates IRF3-PIN1 interaction, which antagonizes IRF3 ubiquitination and degradation, and boosts the antiviral response. Catalyzes ISGylation of influenza A viral NS1 which attenuates virulence; ISGylated NS1 fails to form homodimers and thus to interact with its RNA targets. Catalyzes ISGylation of papillomavirus type 16 L1 protein which results in dominant- negative effect on virus infectivity. Physically associated with polyribosomes, broadly modifies newly synthesized proteins in a cotranslational manner. In an interferon-stimulated cell, newly translated viral proteins are primary targets of ISG15.
UniProt Protein Details:

Protein type:Ligase; EC 6.3.2.-; Ubiquitin conjugating system; Ubiquitin ligase

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: cytosol; perinuclear region of cytoplasm

Molecular Function:ISG15 conjugating enzyme activity; ligase activity; protein binding; ubiquitin-protein ligase activity

Biological Process: cytokine and chemokine mediated signaling pathway; defense response to virus; innate immune response; ISG15-protein conjugation; negative regulation of interferon type I production; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of cyclin-dependent protein kinase activity; regulation of defense response to virus

NCBI Summary:This gene is a member of the HERC family of ubiquitin ligases and encodes a protein with a HECT domain and five RCC1 repeats. Pro-inflammatory cytokines upregulate expression of this gene in endothelial cells. The protein localizes to the cytoplasm and perinuclear region and functions as an interferon-induced E3 protein ligase that mediates ISGylation of protein targets. The gene lies in a cluster of HERC family genes on chromosome 4. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UII4
NCBI GenInfo Identifier:296434523
NCBI Gene ID:51191
NCBI Accession:Q9UII4.2
UniProt Secondary Accession:Q9UII4,Q69G20, B2RTQ1,
UniProt Related Accession:Q9UII4
Molecular Weight:116,852 Da
NCBI Full Name:E3 ISG15--protein ligase HERC5
NCBI Synonym Full Names:HECT and RLD domain containing E3 ubiquitin protein ligase 5
NCBI Official Symbol:HERC5��
NCBI Official Synonym Symbols:CEB1; CEBP1��
NCBI Protein Information:E3 ISG15--protein ligase HERC5
UniProt Protein Name:E3 ISG15--protein ligase HERC5
UniProt Synonym Protein Names:Cyclin-E-binding protein 1; HECT domain and RCC1-like domain-containing protein 5
Protein Family:E3 ISG15--protein ligase
UniProt Gene Name:HERC5��
UniProt Entry Name:HERC5_HUMAN

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