Description
Product Name: | Human Hepatitis B Virus (HBV) Surface Antibody ELISA Kit |
SKU: | HDES0083 |
Size: | 48T |
Target: | HBs-Ab |
Detection Method: | Sandwich ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.6%, Specificity 99.3% |
Sample Type: | Serum, Plasma |
Assay Time: | 45 min |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Sandwich-ELISA as the method to detect the anti - HBs in human serum and plasma. The ELISA Microtiter plate provided in this kit has been pre-coated with purified Hepatitis B Virus Surface (HBs) antigen. Samples are added to the ELISA Microtiter plate wells and the HBs antibody in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugated Mouse-anti-human antibody is added to each well and react with the compound to form “HBs antigen- HBs antibody-HRP antigen-” compound. The substrate reagent is added to initiate the color developing reaction. The presence of anti - HBs can be determined by measuring the absorbance value by using a microplate reader with 450 nm (630 nm) wavelength.