Human Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) ELISA Kit
The Human Heparan Sulfate 6-O-Sulfotransferase 3 (HS6ST3) ELISA Kit is a powerful tool for measuring the levels of HS6ST3 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.HS6ST3 is an enzyme that plays a crucial role in the modification of heparan sulfate, a key component of the extracellular matrix. It is involved in regulating various biological processes including cell adhesion, growth factor signaling, and inflammation.
Understanding the levels of HS6ST3 can provide valuable insights into the mechanisms underlying diseases such as cancer, diabetes, and cardiovascular disorders.With its advanced technology and reliable performance, the Human HS6ST3 ELISA Kit is an essential tool for researchers studying heparan sulfate biology and its implications in human health. Start uncovering new discoveries with this cutting-edge kit today.
Product Name:
Human Heparan Sulfate-6-O-Sulfotransferase 3 (HS6ST3) ELISA Kit
Product Code:
HUDL01369
Size:
96 Assays
Target:
HS6ST3
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
