Human HBsAg (Hepatitis B surface antigen) ELISA Kit
The Human HBsAg (Hepatitis B Surface Antigen) ELISA Kit is a specialized assay designed for the quantitative detection of Hepatitis B surface antigen levels in various biological samples. HBsAg, a critical biomarker for Hepatitis B infection, serves as a key indicator in the diagnosis and monitoring of Hepatitis B virus activity.
Accurate measurement of HBsAg levels is vital for diagnosing Hepatitis B infection, assessing disease progression, and evaluating treatment efficacy. This ELISA kit guarantees exceptional sensitivity and specificity, ensuring accurate and reproducible results for precise quantification of HBsAg. Manufactured under stringent quality control standards, the kit delivers robust performance, making it an ideal choice for both research studies and clinical diagnostics. Trust in the Human HBsAg ELISA Kit for dependable and accurate quantification of this essential viral marker in your investigative endeavors.
Product Name:
Human HBsAg (Hepatitis B surface antigen) ELISA Kit
SKU:
AEES00116
Target:
Human HBsAg (Hepatitis B surface antigen)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human HBsAg. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HBsAg and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HBsAg, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human HBsAg. You can calculate the concentration of Human HBsAg in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-107
94-103
89-101
Average (%)
99
98
96
1:4
Range (%)
95-110
96-106
97-104
Average (%)
102
103
100
1:8
Range (%)
91-105
95-103
90-101
Average (%)
98
98
97
1:16
Range (%)
89-104
99-108
102-109
Average (%)
96
104
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-93
90
EDTA plasma (n=5)
86-98
91
Cell culture media (n=5)
86-96
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.83
2.13
9.87
0.86
2.42
9.2
Standard deviation
0.05
0.1
0.42
0.05
0.12
0.5
C V (%)
6.02
4.69
4.26
5.81
4.96
5.43
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human HBsAg concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HBsAg in samples. No significant cross-reactivity or interference between Human HBsAg and analogues was observed.
