The Human HBC (Hemoglobin C) CLIA Kit is specifically designed for the precise measurement of Hemoglobin C levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results, making it an invaluable tool for various research purposes.Hemoglobin C is an important hemoglobin variant that can lead to a rare type of hemolytic anemia when present in high levels.
Understanding the levels of Hemoglobin C in samples can provide critical insights into various blood disorders and conditions, making it a vital biomarker for diagnostic and research purposes.Overall, the Human HBC CLIA Kit is a reliable and efficient solution for studying Hemoglobin C levels in different biological samples, allowing researchers to further explore the role of this protein in various diseases and conditions.
Product Name:
Human HbC (Hemoglobin C) CLIA Kit
SKU:
HUES01146
Target:
Human HbC (Hemoglobin C)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HbC. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HbC and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HbC, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HbC. You can calculate the concentration of Human HbC in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-105
100-114
86-98
Average (%)
99
108
91
1:4
Range (%)
100-115
97-108
100-115
Average (%)
108
103
106
1:8
Range (%)
99-113
87-100
88-103
Average (%)
105
92
95
1:16
Range (%)
100-117
92-107
97-112
Average (%)
107
99
105
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-108
101
EDTA plasma (n=5)
96-108
102
Cell culture media (n=5)
87-97
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
2.05
8.94
1.0
1.96
8.7
Standard deviation
0.09
0.23
0.73
0.09
0.18
1.0
C V (%)
8.65
11.22
8.17
9.0
9.18
11.49
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HbC concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HbC in samples. No significant cross-reactivity or interference between Human HbC and analogues was observed.
