Similarly, the Human HB Beta (Hemoglobin Beta) CLIA Kit is a reliable tool for the quantitative measurement of HB Beta levels in human samples. This kit is highly sensitive and specific, offering accurate and reproducible results for various research applications. Hemoglobin Beta is a key component of hemoglobin, the protein responsible for transporting oxygen in the blood. Abnormal levels of HB Beta can indicate conditions such as sickle cell anemia, thalassemia, and other hemoglobin disorders.
Monitoring HB Beta levels can provide valuable insights into these conditions and aid in diagnosis and treatment planning. With its ease of use and high-performance capabilities, the Human HB Beta CLIA Kit is a valuable asset for researchers studying hemoglobin disorders and their implications for human health. Get accurate and reliable results with this advanced kit from Assay Genie.
Product Name:
Human HB beta (Hemoglobin Beta) CLIA Kit
SKU:
HUES01147
Target:
Human HB beta (Hemoglobin Beta)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
0.19 μg/mL
Detection range:
0.31-20 μg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HBβ. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HBβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HBβ, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human HBβ. You can calculate the concentration of Human HBβ in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
102-112
95-111
86-99
Average (%)
107
103
92
1:4
Range (%)
91-105
85-98
93-109
Average (%)
98
92
99
1:8
Range (%)
95-108
94-107
100-114
Average (%)
101
99
106
1:16
Range (%)
89-102
102-118
95-111
Average (%)
96
110
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
91
EDTA plasma (n=5)
89-102
96
Cell culture media (n=5)
94-108
100
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.98
3.03
8.01
1.03
2.84
7.54
Standard deviation
0.09
0.35
0.64
0.13
0.22
0.81
C V (%)
9.18
11.55
7.99
12.62
7.75
10.74
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human HBβ concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human HBβ in samples. No significant cross-reactivity or interference between Human HBβ and analogues was observed.
