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Human HABP1 / Hyaluronan Binding Protein 1 ELISA Kit

SKU:
HUFI00829
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q07021
Sensitivity:
0.094ng/ml
Range:
0.156-10ng/ml
ELISA Type:
Sandwich
Synonyms:
HABP1, C1QBP, Hyaluronan Binding Protein 1, gC1qBP, gC1qR, SF2p32, p32, Glycoprotein gC1qBP, HABP1p33, GC1q-R protein, C1q globular domain-binding protein, gC1Q-R, Hyaluronan-binding protein 1, Mitochondrial matrix protein p32, splicing factor SF2-as
Reactivity:
Human
Research Area:
Immunology
€599
Frequently bought together:

Description

Human HABP1 / Hyaluronan Binding Protein 1 ELISA Kit

Hyaluronan Binding Protein 1 (HABP1) is a tumor-promoting protein, which is primarily associated with breast cancer. It has been shown to bind to hyaluronic acid and has been found to be present in over 100 types of tumors, including lung, breast, and ovarian cancers. HABP1 also plays a key role in the development of kidney disease. The Assay Genie Human HABP1 ELISA kit is a highly sensitive assay for the quantitative measurement of HABP1 in serum, blood, plasma, cell culture supernatant, and tissue samples.

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human HABP1 / Hyaluronan Binding Protein 1 ELISA Kit
Product Code:HUFI00829
Size:96 Assays
Alias:HABP1, C1QBP, Hyaluronan Binding Protein 1, gC1qBP, gC1qR, SF2p32, p32, Glycoprotein gC1qBP, HABP1p33, GC1q-R protein, C1q globular domain-binding protein, gC1Q-R, Hyaluronan-binding protein 1, Mitochondrial matrix protein p32, splicing factor SF2-associated protein
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human HABP1/C1QBP concentrations in serum plasma and other biological fluids.
Sensitivity:0.094ng/ml
Range:0.156-10ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human HABP1/C1QBP and the recovery rates were calculated by comparing the measured value to the expected amount of Human HABP1/C1QBP in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)85-10094
EDTA plasma(n=5)89-10597
UFH plasma(n=5)88-10094
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human HABP1/C1QBP and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-98%91-105%86-97%
EDTA plasma(n=5)82-101%90-101%84-100%
UFH plasma(n=5)82-100%84-95%82-91%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ07021
UniProt Protein Function:C1QBP: a multifunctional and multicompartmental protein involved in inflammation and infection processes, ribosome biogenesis, regulation of apoptosis, transcriptional regulation and pre-mRNA splicing. Originally identified via its binding interactions with Splicing Factor (SF-2). Multiple, diverse binding partners of C1QBP were subsequently identified, including the globular heads of complement component C1q, hyaluronic acid, selected protein kinases, the tumor suppressor ARF, and multiple antigens of bacterial and viral origin. Overexpressed in a number of cancer cell types, and has been implicated in the Warburg effect, whereby cancer cells shift their metabolism from oxidative phosphorylation to glycolysis. Inhibits the Mitochondrial Permeability Transition (MPT) pore, possibly serving a protective function against damage from oxidative stress. Binding to C1q inhibits C1. In complex with cytokeratin-1/KRT1 is a high affinty receptor for kininogen-1/HMWK. The secreted form may enhance both extrinsic and intrinsic coagulation pathways. The cell surface form may require docking with transmembrane proteins for downstream signaling which might be specific for a cell-type or response. Belongs to the MAM33 family.
UniProt Protein Details:Protein type:Motility/polarity/chemotaxis; Receptor, misc.; Nucleolus; Mitochondrial

Chromosomal Location of Human Ortholog: 17p13.3

Cellular Component: cell surface; cytoplasm; cytosol; membrane; mitochondrion; nucleus; plasma membrane

Molecular Function:adrenergic receptor binding; complement component C1q binding; hyaluronic acid binding; kininogen binding; mRNA binding; protein binding; transcription corepressor activity; transcription factor binding

Biological Process: blood coagulation, intrinsic pathway; immune response; mature ribosome assembly; negative regulation of defense response to virus; negative regulation of interferon-gamma production; negative regulation of interleukin-12 production; negative regulation of nuclear mRNA splicing, via spliceosome; negative regulation of transcription from RNA polymerase II promoter; phosphoinositide 3-kinase cascade; positive regulation of apoptosis; positive regulation of cell adhesion; positive regulation of protein kinase B signaling cascade; regulation of complement activation

NCBI Summary:The human complement subcomponent C1q associates with C1r and C1s in order to yield the first component of the serum complement system. The protein encoded by this gene is known to bind to the globular heads of C1q molecules and inhibit C1 activation. This protein has also been identified as the p32 subunit of pre-mRNA splicing factor SF2, as well as a hyaluronic acid-binding protein. [provided by RefSeq, Jul 2008]
UniProt Code:Q07021
NCBI GenInfo Identifier:730772
NCBI Gene ID:708
NCBI Accession:Q07021.1
UniProt Secondary Accession:Q07021,Q2HXR8, Q9NNY8,
UniProt Related Accession:Q07021
Molecular Weight:31,362 Da
NCBI Full Name:Complement component 1 Q subcomponent-binding protein, mitochondrial
NCBI Synonym Full Names:complement C1q binding protein
NCBI Official Symbol:C1QBP
NCBI Official Synonym Symbols:p32; HABP1; gC1qR; GC1QBP; SF2p32; gC1Q-R
NCBI Protein Information:complement component 1 Q subcomponent-binding protein, mitochondrial
UniProt Protein Name:Complement component 1 Q subcomponent-binding protein, mitochondrial
UniProt Synonym Protein Names:ASF/SF2-associated protein p32; Glycoprotein gC1qBP; C1qBP; Hyaluronan-binding protein 1; Mitochondrial matrix protein p32; gC1q-R protein; p33
Protein Family:Complement component 1 Q subcomponent-binding protein
UniProt Gene Name:C1QBP
UniProt Entry Name:C1QBP_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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