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Human Guanylate Cyclase Activator 2A (GUCA2A) ELISA Kit

SKU:
HUDL01283
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q02747
Sensitivity:
8.78fmol/mL
Range:
31.25-2000fmol/mL
ELISA Type:
Sandwich
Synonyms:
GUCA2,STARA,GCAP-I,Guanylin,Guanylate cyclase-activating protein 1
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Guanylate Cyclase Activator 2A (GUCA2A) ELISA Kit

The Human Guanylate Cyclase Activator 2A (GUCA2A) ELISA Kit is specifically designed for the accurate and precise measurement of GUCA2A levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures reliable and reproducible results, making it an essential tool for researchers in a variety of fields.GUCA2A is a key activator of guanylate cyclase, a crucial enzyme involved in various physiological processes such as smooth muscle relaxation and regulation of blood pressure.

Dysregulation of GUCA2A has been linked to diseases like hypertension and gastrointestinal disorders, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.Overall, the Human GUCA2A ELISA Kit offers researchers a valuable tool for investigating the role of GUCA2A in human physiology and disease, providing valuable insights that can drive advancements in healthcare and medicine.

Product Name:Human Guanylate Cyclase Activator 2A (GUCA2A) ELISA Kit
Product Code:HUDL01283
Size:96 Assays
Target:GUCA2A
Synonyms:GUCA2, STARA, GCAP-I, Guanylin, Guanylate cyclase-activating protein 1
Detection Method:Sandwich
Range:31.25-2000fmol/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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