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Human GTF2A1 Recombinant Protein (RPPB3645)

SKU:
RPPB3645
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P52655
$328
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Description

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Product Name:Human GTF2A1 Recombinant Protein
Product Code:RPPB3645
Size:10µg
Species:Human
Target:GTF2A1
Synonyms:General transcription factor IIA, 1, 19/37kDa, TF2A1, TFIIA, TFIIA-42, TFIIAL, Transcription initiation factor IIA subunit 1, Transcription initiation factor TFIIA 42 kDa subunit, GTF2A1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:The GTF2A1 solution (0.5mg/ml) contains 20mM Tris-HCl(pH8.0), 20% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMANSANT NTVPKLYRSV IEDVINDVRD IFLDDGVDEQ VLMELKTLWE NKLMQSRAVD GFHSEEQQLL LQVQQQHQPQ QQQHHHHHHH QQAQPQQTVP QQAQTQQVLI PASQQATAPQ VIVPDSKLIQ HMNASNMSAA ATAATLALPA GVTPVQQILT NSGQLLQVVR AANGAQYIFQ PQQSVVLQQQ VIPQMQPGGV QAPVIQQVLA PLPGGISPQT GVIIQPQQIL FTGNKTQVIP TTVAAPTPAQ AQITATGQQQ PQAQPAQTQA PLVLQVD

General Transcription Factor IIA, 1 (GTF2A1) in a complex along with TBP mediates transcriptional activity. GTF2A1 is a part of the transcription machinery of RNA polymerase II and is also participates in transcriptional activation.

GTF2A1 Human Recombinant produced in E. coli is. a single polypeptide chain containing 297 amino acids (1-274) and having a molecular mass of 32.2kDa. GTF2A1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GTF2A1: a general transcription factor that plays an important role in transcriptional activation. A component of the transcription machinery of RNA polymerase II. Phosphorylated by Taf(II) 250 on serines that regulate TBP binding. Interacts with TBP (the TATA-binding protein). Two isoforms, 42 kDa and 37 kDa, are produced by alternative initiation.
UniProt Protein Details:

Protein type:Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 14q31.1

Cellular Component: cytoplasm; nucleoplasm; transcription factor TFIIA complex

Molecular Function:DNA binding; protein binding; protein heterodimerization activity; TATA-binding protein binding; transcription coactivator activity; transcription factor binding

Biological Process: RNA elongation from RNA polymerase II promoter; snRNA transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter

NCBI Summary:Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and several general initiation factors (summarized by DeJong and Roeder, 1993 [PubMed 8224848]). One of these factors is TFIIA, which when purified from HeLa extracts consists of 35-, 19-, and 12-kD subunits.[supplied by OMIM, Jul 2010]
UniProt Code:P52655
NCBI GenInfo Identifier:1711663
NCBI Gene ID:2957
NCBI Accession:P52655.1
UniProt Secondary Accession:P52655,Q3KNQ9,
UniProt Related Accession:P52655
Molecular Weight:37,152 Da
NCBI Full Name:Transcription initiation factor IIA subunit 1
NCBI Synonym Full Names:general transcription factor IIA subunit 1
NCBI Official Symbol:GTF2A1��
NCBI Official Synonym Symbols:TF2A1; TFIIA; TFIIAL; TFIIA-42��
NCBI Protein Information:transcription initiation factor IIA subunit 1
UniProt Protein Name:Transcription initiation factor IIA subunit 1
UniProt Synonym Protein Names:General transcription factor IIA subunit 1; TFIIAL; Transcription initiation factor TFIIA 42 kDa subunit; TFIIA-42
Protein Family:Transcription initiation factor
UniProt Gene Name:GTF2A1��
UniProt Entry Name:TF2AA_HUMAN

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