Human GSTT1 Recombinant Protein (RPPB1777)
- SKU:
- RPPB1777
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P30711
- Research Area:
- Enzymes
Description
| Product Name: | Human GSTT1 Recombinant Protein |
| Product Code: | RPPB1777 |
| Size: | 20µg |
| Species: | Human |
| Target: | GSTT1 |
| Synonyms: | Glutathione S-transferase theta-1, GST class-theta-1, Glutathione transferase T1-1, GSTT1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GSTT1 solution contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMGL ELYLDLLSQP CRAVYIFAKK NDIPFELRIV DLIKGQHLSD ACAQVNPLKK VPALKDGDFT LTESVAILLY LTRKYKVPDY WYPQDLQARA RVDEYLAWQH TTLRRSCLRA LWHKVMFPVF LGEPVSPQTL AATLAELDVT LQLLEDKFLQ NKAFLTGPHI SLADLVAITE LMHPVGAGCQ VFEGRPKLAT WRQRVEAAVG EDLFQEAHEV ILKAKDFPPA DPTIKQKLMP WVLAMIR |
GSTT1 belongs to a superfamily of proteins which catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. GSTT1 is one of the GSTs� four main classes: alpha, mu, pi and theta (which includes GSTT1 and GSTT2). GSTT1 is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, such as epoxybutane, ethylene oxides, halomethane with glutathione. GSTT1 is found in erythrocytes, at low levels in the liver as well as in Clara and ciliated cells at the alveolar/bronchiolar junction in the lung.The GSTT1 gene is deficient in 38% of the population. The GSTTI enzyme deficiency might influence the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. The presence or absence of the GSTT1 gene is concurrent with GSST1+ (the conjugator) and GSTT1- (the non-conjugator) phenotypes correspondingly. The GSTT1+ phenotype is able to catalyze the glutathione conjugation of dichloromethane. GSTT1-null genotypes are seen as having a higher risk of developing leukoplakia. Germline genetic polymorphism in GSTT1 is linked to breast cancer.
GSTT1 Human Recombinant fused with 37 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 277 amino acids (1-240 a.a.) and having a molecular mass of 31.5kDa.The GSTT1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family. |
| UniProt Protein Details: | Protein type:Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; Transferase; EC 2.5.1.18 Chromosomal Location of Human Ortholog: 22q11.23 Cellular Component: cytosol Molecular Function:glutathione peroxidase activity; glutathione transferase activity Biological Process: glutathione metabolic process |
| NCBI Summary: | The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| UniProt Code: | P30711 |
| NCBI GenInfo Identifier: | 21264427 |
| NCBI Gene ID: | 2952 |
| NCBI Accession: | P30711.4 |
| UniProt Secondary Accession: | P30711,O00226, Q5TZY2, Q6IC69, Q969K8, Q96IY3, |
| UniProt Related Accession: | P30711 |
| Molecular Weight: | 13,517 Da |
| NCBI Full Name: | Glutathione S-transferase theta-1 |
| NCBI Synonym Full Names: | glutathione S-transferase theta 1 |
| NCBI Official Symbol: | GSTT1�� |
| NCBI Protein Information: | glutathione S-transferase theta-1 |
| UniProt Protein Name: | Glutathione S-transferase theta-1 |
| UniProt Synonym Protein Names: | GST class-theta-1; Glutathione transferase T1-1 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTT1�� |
| UniProt Entry Name: | GSTT1_HUMAN |
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