Human GSTP1 Recombinant Protein (RPPB1773)
- SKU:
- RPPB1773
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P09211
- Research Area:
- Enzymes
Description
| Product Name: | Human GSTP1 Recombinant Protein |
| Product Code: | RPPB1773 |
| Size: | 20µg |
| Species: | Human |
| Target: | GSTP1 |
| Synonyms: | Glutathione S-transferase P, GST class-pi, GSTP1-1, GSTP1, FAEES3, GST3, PI, DFN7. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GSTP1 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH7.0), 30% glycerol, 1mM EDTA and 0.1mM PMSF. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMPPY TVVYFPVRGR CAALRMLLAD QGQSWKEEVV TVETWQEGSL KASCLYGQLP KFQDGDLTLY QSNTILRHLG RTLGLYGKDQ QEAALVDMVN DGVEDLRCKY ISLIYTNYEA GKDDYVKALP GQLKPFETLL SQNQGGKTFI VGDQISFADY NLLDLLLIHE VLAPGCLDAF PLLSAYVGRL SARPKLKAFL ASPEYVNLPI NGNGKQ |
| Biological Activity: | Specific activity is > 80 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25�C. |
GSTP1 is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are believed to function in xenobiotic metabolism and have a part in susceptibility to cancer, and other diseases. GSTP1 is a glutathione S-transferase belonging to the pi class. GST family enzymes play a significant role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based upon the biochemical, immunologic and structural properties of the soluble GSTs they are grouped into 4 main classes: alpha, mu, pi, and theta. The GSTP1 enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form a Sulfur-substituted glutathione. The reactions employing glutathione contribute the transformation of a broad range of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress.The GSTP1 inactivation through CpG hypermethylation is frequent in pituitary adenomas and may be a factor in aggressive pituitary tumor behavior. GSTP1 is may be a transcriptional target of the p53 tumor suppressor gene. Single-nucleotide polymorphism in GSTP1 is linked to modified protein binding, which influence GSTP1's contribution to carcinogen and drug metabolism, and possibly disease pathogenesis and/or drug response. GST-pi might have central roles in proliferation of androgen-independent human prostate cancer cells.
GSTP1 Human Recombinant fused with 36 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 246 amino acids(1-210 a.a.) And having a molecular mass of 27.4kDa.The GSTP1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family. |
| UniProt Protein Details: | Protein type:Transferase; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione Chromosomal Location of Human Ortholog: 11q13 Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; cytosol; nucleus; vesicle Molecular Function:JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity Biological Process: negative regulation of MAP kinase activity; negative regulation of JNK activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; negative regulation of biosynthetic process; response to reactive oxygen species; glutathione metabolic process; negative regulation of nitric-oxide synthase biosynthetic process; negative regulation of fibroblast proliferation; positive regulation of superoxide release; xenobiotic metabolic process; negative regulation of protein kinase activity; negative regulation of apoptosis |
| NCBI Summary: | Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P09211 |
| NCBI GenInfo Identifier: | 121746 |
| NCBI Gene ID: | 2950 |
| NCBI Accession: | P09211.2 |
| UniProt Secondary Accession: | P09211,O00460, Q15690, Q5TZY3, |
| UniProt Related Accession: | P09211 |
| Molecular Weight: | 23,356 Da |
| NCBI Full Name: | Glutathione S-transferase P |
| NCBI Synonym Full Names: | glutathione S-transferase pi 1 |
| NCBI Official Symbol: | GSTP1�� |
| NCBI Official Synonym Symbols: | PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22�� |
| NCBI Protein Information: | glutathione S-transferase P; GSTP1-1; GST class-pi; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III |
| UniProt Protein Name: | Glutathione S-transferase P |
| UniProt Synonym Protein Names: | GST class-pi; GSTP1-1 |
| Protein Family: | Glutathione S-transferase |
| UniProt Gene Name: | GSTP1�� |
| UniProt Entry Name: | GSTP1_HUMAN |
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