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Human GSTM2 / Glutathione S Transferase Mu 2, Muscle ELISA Kit (HUFI02536)

SKU:
HUFI02536
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P28161
Sensitivity:
0.469ng/ml
Range:
0.781-50ng/ml
ELISA Type:
Sandwich
Synonyms:
GSTMu2, GSTm2, GST class-mu 2, GSTM2-2
Reactivity:
Human
Research Area:
Cell Biology
€599
Frequently bought together:

Description

Human GSTM2/Glutathione S Transferase Mu 2, Muscle ELISA Kit

The Human GSTM2 (Glutathione S-Transferase Mu 2 Muscle) ELISA Kit is designed for the precise measurement of GSTM2 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for various research purposes.GSTM2 is an important enzyme involved in detoxification processes within the body, particularly in the metabolism of xenobiotics and carcinogens.

It plays a crucial role in protecting cells from damage caused by harmful substances, making it a valuable biomarker for studying conditions such as cancer, oxidative stress, and drug metabolism. This ELISA kit provides researchers with a reliable tool for investigating the role of GSTM2 in health and disease, as well as potential therapeutic interventions.

Product Name:Human GSTM2 / Glutathione S Transferase Mu 2, Muscle ELISA Kit
Product Code:HUFI02536
Size:96 Assays
Alias:GST?2, GSTm2, GST class-mu 2, GSTM2-2
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human GST?2/GSTm2 concentrations in serum plasma and other biological fluids.
Sensitivity:0.469ng/ml
Range:0.781-50ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human GST?2/GSTm2 and the recovery rates were calculated by comparing the measured value to the expected amount of Human GST?2/GSTm2 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)86-10499
EDTA plasma(n=5)87-10396
UFH plasma(n=5)86-10292
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human GST?2/GSTm2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)89-105%88-96%87-101%
EDTA plasma(n=5)82-101%90-97%82-94%
UFH plasma(n=5)82-95%80-89%87-96%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotP28161
UniProt Protein Function:GSTM2: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family.
UniProt Protein Details:Protein type:Transferase; EC 2.5.1.18; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 1p13.3

Cellular Component: sarcoplasmic reticulum; cytoplasm; cytosol

Molecular Function:protein homodimerization activity; enzyme binding; glutathione transferase activity; glutathione binding; receptor binding

Biological Process: regulation of skeletal muscle contraction via regulation of the release of sequestered calcium ion; glutathione metabolic process; nitrobenzene metabolic process; xenobiotic metabolic process; xenobiotic catabolic process

NCBI Summary:Cytosolic and membrane-bound forms of glutathione S-transferase are encoded by two distinct supergene families. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: alpha, kappa, mu, omega, pi, sigma, theta and zeta. This gene encodes a glutathione S-transferase that belongs to the mu class. The mu class of enzymes functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. The genes encoding the mu class of enzymes are organized in a gene cluster on chromosome 1p13.3 and are known to be highly polymorphic. These genetic variations can change an individual's susceptibility to carcinogens and toxins as well as affect the toxicity and efficacy of certain drugs. [provided by RefSeq, Jul 2008]
UniProt Code:P28161
NCBI GenInfo Identifier:232204
NCBI Gene ID:2946
NCBI Accession:P28161.2
UniProt Secondary Accession:P28161,Q2M318, Q5TZY5, Q8WWE1, B4DRY4, E9PEM9,
UniProt Related Accession:P28161
Molecular Weight:218
NCBI Full Name:Glutathione S-transferase Mu 2
NCBI Synonym Full Names:glutathione S-transferase mu 2 (muscle)
NCBI Official Symbol:GSTM2
NCBI Official Synonym Symbols:GST4; GSTM; GTHMUS; GSTM2-2
NCBI Protein Information:glutathione S-transferase Mu 2; GST, muscle; GST class-mu 2; glutathione S-transferase 4; glutathione S-transferase M1; glutathione S-aryltransferase M2; glutathione S-alkyltransferase M2; glutathione S-aralkyltransferase M2; S-(hydroxyalkyl)glutathione lyase M2; glutathione S-transferase M2 (muscle)
UniProt Protein Name:Glutathione S-transferase Mu 2
UniProt Synonym Protein Names:GST class-mu 2; GSTM2-2
Protein Family:Glutathione S-transferase
UniProt Gene Name:GSTM2
UniProt Entry Name:GSTM2_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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