Human GSTα1 (Glutathione S Transferase Alpha 1) ELISA Kit
The Human GSTα1 (Glutathione S Transferase Alpha 1) ELISA Kit is meticulously designed for the precise and quantitative assessment of GSTα1 protein levels in diverse human biological samples. GSTα1, a critical detoxification enzyme, plays a key role in catalyzing the conjugation of reduced glutathione to various substrates, aiding in the detoxification and elimination of harmful compounds from cells. Accurate measurement of GSTα1 using our ELISA kit provides valuable insights into cellular detoxification mechanisms, xenobiotic metabolism, and oxidative stress responses.
With exceptional sensitivity and specificity, our ELISA kit ensures reliable and reproducible results. Manufactured under strict quality control standards, this kit delivers robust performance and user-friendly protocols, making it an ideal choice for both research and clinical applications. Rely on our Human GSTα1 ELISA Kit for precise and dependable quantification of this essential enzyme in your studies.
Product Name:
Human GSTα1 (Glutathione S Transferase Alpha 1) ELISA Kit
SKU:
AEES00106
Target:
Human GSTα1 (Glutathione S Transferase Alpha 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GSTα1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GSTα1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GSTα1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GSTα1. You can calculate the concentration of Human GSTα1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
92-104
94-102
92-106
Average (%)
97
98
99
1:4
Range (%)
96-109
97-109
94-105
Average (%)
103
102
100
1:8
Range (%)
88-95
93-108
91-101
Average (%)
92
100
96
1:16
Range (%)
92-106
100-112
97-108
Average (%)
100
104
100
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-111
103
EDTA plasma (n=5)
89-101
94
Cell culture media (n=5)
101-111
104
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.86
2.28
8.66
0.9
2.0
8.79
Standard deviation
0.05
0.11
0.44
0.05
0.09
0.35
C V (%)
5.81
4.82
5.08
5.56
4.5
3.98
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GSTα1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GSTα1 in samples. No significant cross-reactivity or interference between Human GSTα1 and analogues was observed.
