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Human Growth Hormone Releasing Hormone (GHRH) ELISA Kit

SKU:
HUDL01184
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01286
Sensitivity:
6.15pg/mL
Range:
15.625-1000pg/mL
ELISA Type:
Competitive
Synonyms:
GRF,GHRF,Somatocrinin,Growth-Hormone-Releasing Factor,Sermorelin,Somatoliberin
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

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Human Growth Hormone Releasing Hormone (GHRH) ELISA Kit

The Human Growth Hormone Releasing Hormone (GHRH) ELISA Kit is specifically designed for the accurate measurement of GHRH levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers dependable and consistent results, making it an excellent choice for various research applications.GHRH is a vital hormone that plays a crucial role in regulating the production and secretion of growth hormone in the body. Imbalances in GHRH levels can lead to growth disorders and other health complications.

By accurately measuring GHRH levels, researchers can gain valuable insights into growth-related conditions and potential treatment options.Whether studying growth disorders, hormone regulation, or exploring potential therapeutic interventions, the Human GHRH ELISA Kit provides a reliable tool for researchers seeking to deepen their understanding of growth hormone regulation and related health conditions.

Product Name:Human Growth Hormone Releasing Hormone (GHRH) ELISA Kit
Product Code:HUDL01184
Size:96 Assays
Target:GHRH
Synonyms:GRF GHRF Somatocrinin Growth-Hormone-Releasing Factor Sermorelin Somatoliberin
Detection Method:Competition
Range:15.625-1000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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