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Human GroES Recombinant Protein (RPPB5224)

SKU:
RPPB5224
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P61604
€309
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Description

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Product Name:Human GroES Recombinant Protein
Product Code:RPPB5224
Size:10µg
Species:Human
Target:GroES
Synonyms:CPN10, GROES, HSP10, HSPE1, Chaperonin-10, 10 kDa heat shock protein mitochondrial, 10 kDa chaperonin, Early-pregnancy factor, EPF.
Source:Escherichia Coli
Formulation:GroES His Human was filtered (0.4�m) and lyophilized from 0.5 mg/ml supplied in 0.05M phosphate buffer, 0.075 M NaCl, pH 7.4.
Solubility:It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it on cell culture.
Stability:Store lyophilized GroES His at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted GroES His can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MKHHHHHHAS AGQAFRKFLP LFDRVLVERS AAETVTKGGI MLPEKSQGKV LQATVVAVGS GSKGKGGEIQ PVSVKVGDKV LLPEYGGTKV VLDDKDYFLF RDGDILGKYV D

HSP10 is part of the molecular chaperons, that are crucial for thir efficient folding of proteins in normal as well as stress conditions. GroES function is to bind to HSP60 in the presence of ATP, thus causing a change in the HSP60 conformation & enclosing the protein substrate within the complex. ATP hydrolysis by chaperonin-60 which destabilizes the HSP10-HSP60 complex, thereby allowing it to dissociate and secrete the substrate protein. GroES having the NCBI accession number of NP_002148 was purified by using conventional chromatography techniques.

GroES His Protein is 12.0 kDa protein containing 111 amino acid residues of the GroES Human and the 10 aa N-Terminal His-tag.

UniProt Protein Function:HSPE1: Eukaryotic CPN10 homolog which is essential for mitochondrial protein biogenesis, together with CPN60. Binds to CPN60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. Belongs to the GroES chaperonin family.
UniProt Protein Details:

Protein type:Mitochondrial

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: membrane; mitochondrial matrix; mitochondrion

Molecular Function:ATP binding; chaperone binding; protein binding; unfolded protein binding

Biological Process: caspase activation; osteoblast differentiation; protein folding; response to unfolded protein

NCBI Summary:This gene encodes a major heat shock protein which functions as a chaperonin. Its structure consists of a heptameric ring which binds to another heat shock protein in order to form a symmetric, functional heterodimer which enhances protein folding in an ATP-dependent manner. This gene and its co-chaperonin, HSPD1, are arranged in a head-to-head orientation on chromosome 2. Naturally occurring read-through transcription occurs between this locus and the neighboring locus MOBKL3.[provided by RefSeq, Feb 2011]
UniProt Code:P61604
NCBI GenInfo Identifier:47606335
NCBI Gene ID:3336
NCBI Accession:P61604.2
UniProt Secondary Accession:P61604,O95421, Q04984, Q53X54, Q9UDH0,
UniProt Related Accession:P61604
Molecular Weight:10,932 Da
NCBI Full Name:10 kDa heat shock protein, mitochondrial
NCBI Synonym Full Names:heat shock protein family E (Hsp10) member 1
NCBI Official Symbol:HSPE1��
NCBI Official Synonym Symbols:EPF; CPN10; GROES; HSP10��
NCBI Protein Information:10 kDa heat shock protein, mitochondrial
UniProt Protein Name:10 kDa heat shock protein, mitochondrial
UniProt Synonym Protein Names:10 kDa chaperonin; Chaperonin 10; CPN10; Early-pregnancy factor; EPF
UniProt Gene Name:HSPE1��
UniProt Entry Name:CH10_HUMAN

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