Human GR alpha (Glucocorticoid Receptor Alpha) ELISA Kit
The Human GR (Glucocorticoid Receptor) Alpha ELISA Kit is specifically designed for the accurate measurement of GR Alpha levels in human serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures precise and reliable results, making it an invaluable tool for researchers in a variety of fields.The GR Alpha protein plays a critical role in the regulation of gene expression and cellular response to stress hormones, making it a key player in various physiological processes.
Dysregulation of GR Alpha has been linked to conditions such as metabolic disorders, immune system dysfunction, and mood disorders, highlighting its importance as a potential therapeutic target.By using the Human GR Alpha ELISA Kit, researchers can gain valuable insights into the role of GR Alpha in health and disease, paving the way for new treatments and interventions. Trust in this kit for accurate and reproducible results that will advance your research efforts.
Product Name:
Human GR alpha (Glucocorticoid Receptor Alpha) ELISA Kit
SKU:
HUES02931
Target:
Human GR alpha (Glucocorticoid Receptor Alpha)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GRα. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRα, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GRα. You can calculate the concentration of Human GRα in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
96-109
97-113
89-100
Average (%)
101
103
95
1:4
Range (%)
92-105
83-97
84-98
Average (%)
97
90
91
1:8
Range (%)
93-103
85-97
83-96
Average (%)
98
90
88
1:16
Range (%)
89-102
82-93
84-99
Average (%)
95
87
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
85-100
92
EDTA plasma (n=5)
84-98
91
Cell culture media (n=5)
89-100
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.06
3.08
8.11
1.1
3.14
7.35
Standard deviation
0.07
0.13
0.25
0.07
0.15
0.29
C V (%)
6.6
4.22
3.08
6.36
4.78
3.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GRα concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GRα in samples. No significant cross-reactivity or interference between Human GRα and analogues was observed.
