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Human GPX3 Recombinant Protein (RPPB1745)

SKU:
RPPB1745
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P22352
Research Area:
Enzymes
€309
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Description

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Product Name:Human GPX3 Recombinant Protein
Product Code:RPPB1745
Size:10µg
Species:Human
Target:GPX3
Synonyms:Glutathione peroxidase 3, GPx-3, GSHPx-3, Extracellular glutathione peroxidase, Plasma glutathione peroxidase, GPx-P, GSHPx-P, GPX3, GPXP.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The GPX3 solution contains 20mM Tris-HCl buffer (pH7.5), 40% glycerol, 0.15M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MQSRGQEKSK MDCHGGISGT IYEYGALTID GEEYIPFKQY AGKYVLFVNV ASYCGLTGQY IELNALQEEL APFGLVILGF PCNQFGKQEP GENSEILPTL KYVRPGGGFV PNFQLFEKGD VNGEKEQKFY TFLKNSCPPT SELLGTSDRL FWEPMKVHDI RWNFEKFLVG PDGIPIMRWH HRTTVSNVKM DILSYMRRQA ALGVKRK

Glutathione peroxidase 3 (GPX3) is a member of the glutathione peroxidase family, which acts in the detoxification of hydrogen peroxide. GPX3 shields cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. The GPX3 protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.

GPX3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 227 amino acids (21-226) and having a molecular mass of 25.7kDa.GPX3 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.
UniProt Protein Details:

Protein type:Secreted; Other Amino Acids Metabolism - glutathione; Oxidoreductase; Secreted, signal peptide; Lipid Metabolism - arachidonic acid; EC 1.11.1.9

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular region; extracellular space

Molecular Function:glutathione peroxidase activity; protein binding; selenium binding; transcription factor binding

Biological Process: hydrogen peroxide catabolic process; protein homotetramerization; response to lipid hydroperoxide; response to reactive oxygen species

NCBI Summary:This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]
UniProt Code:P22352
NCBI GenInfo Identifier:172046796
NCBI Gene ID:2878
NCBI Accession:P22352.2
UniProt Secondary Accession:P22352,O43787, Q86W78, Q9NZ74, Q9UEL1,
UniProt Related Accession:P22352
Molecular Weight:25,552 Da
NCBI Full Name:Glutathione peroxidase 3
NCBI Synonym Full Names:glutathione peroxidase 3
NCBI Official Symbol:GPX3��
NCBI Official Synonym Symbols:GPx-P; GSHPx-3; GSHPx-P��
NCBI Protein Information:glutathione peroxidase 3
UniProt Protein Name:Glutathione peroxidase 3
UniProt Synonym Protein Names:Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P
Protein Family:Probable glutathione peroxidase
UniProt Gene Name:GPX3��
UniProt Entry Name:GPX3_HUMAN

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