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Human GPT Recombinant Protein (RPPB1827)

SKU:
RPPB1827
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P24298
Research Area:
Enzymes
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Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human GPT Recombinant Protein
Product Code:RPPB1827
Size:3U
Species:Human
Target:GPT
Synonyms:Alanine aminotransferase 1, ALT1, EC 2.6.1.2, Glutamate pyruvate transaminase 1, GPT 1, Glutamic--alanine transaminase 1, Glutamic--pyruvic transaminase 1, GPT, AAT1, GPT1.
Source:Escherichia Coli
Physical Appearance:Sterile liquid formulation.
Formulation:The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5'-phosphate.
Stability:AAT1 although stable at 10°C for 5 days, should be stored below -18°C.Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological Activity:The specific activity was found to be 1,020 U/mg.

Alanine transaminase or ALT is a transaminaseenzyme.ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamatealanine+ a-ketoglutarate= pyruvate+ glutamateIt is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT). Diagnostically, it is almost always measured in units/litre (U/L).

Alanine Aminotransferase Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT.The ALT is purified by proprietary chromatographic techniques.

UniProt Protein Function:GPT: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. Alanine aminotransferase subfamily.
UniProt Protein Details:

Protein type:EC 2.6.1.2; Transferase; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: cytosol

Molecular Function:alanine transaminase activity

Biological Process: amino acid biosynthetic process

NCBI Summary:This gene encodes cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.[provided by RefSeq, Nov 2009]
UniProt Code:P24298
NCBI GenInfo Identifier:46577683
NCBI Gene ID:2875
NCBI Accession:P24298.3
UniProt Secondary Accession:P24298,P78398, Q93076, B0YJ18, D3DWM7,
UniProt Related Accession:P24298
Molecular Weight:54,637 Da
NCBI Full Name:Alanine aminotransferase 1
NCBI Synonym Full Names:glutamic--pyruvic transaminase
NCBI Official Symbol:GPT��
NCBI Official Synonym Symbols:AAT1; ALT1; GPT1��
NCBI Protein Information:alanine aminotransferase 1
UniProt Protein Name:Alanine aminotransferase 1
UniProt Synonym Protein Names:Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
Protein Family:Alanine aminotransferase
UniProt Gene Name:GPT��
UniProt Entry Name:ALAT1_HUMAN

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