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Human G Protein Coupled Receptor 120 (GPR120) ELISA Kit

SKU:
HUDL01240
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q5NUL3
Sensitivity:
0.224ng/mL
Range:
0.625-40ng/mL
ELISA Type:
Sandwich
Synonyms:
GPR129,GT01,PGR4,O3FAR1,Omega-3 fatty acid receptor 1,Free Fatty Acid Receptor 4
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human G Protein Coupled Receptor 120 (GPR120) ELISA Kit

The Human G-Protein Coupled Receptor 120 (GPR120) ELISA Kit is a powerful tool for accurately measuring GPR120 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring dependable and consistent results for a variety of research studies.GPR120 is a G-protein coupled receptor known for its role in regulating metabolic and inflammatory processes in the body.

It is involved in pathways related to obesity, diabetes, and inflammation, making it a valuable biomarker for understanding these conditions and developing targeted therapies.Overall, the Human GPR120 ELISA Kit is an essential tool for researchers looking to investigate the role of GPR120 in various physiological and pathological processes, providing valuable insights into potential therapeutic strategies.

Product Name:Human G Protein Coupled Receptor 120 (GPR120) ELISA Kit
Product Code:HUDL01240
Size:96 Assays
Target:GPR120
Synonyms:GPR129, GT01, PGR4, O3FAR1, Omega-3 fatty acid receptor 1, Free Fatty Acid Receptor 4
Detection Method:Sandwich
Range:0.625-40ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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