Human GPI Recombinant Protein (RPPB1733)
- SKU:
- RPPB1733
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P06744
- Research Area:
- Enzymes
Description
| Product Name: | Human GPI Recombinant Protein |
| Product Code: | RPPB1733 |
| Size: | 20µg |
| Species: | Human |
| Target: | GPI |
| Synonyms: | Glucose-6-phosphate isomerase, Phosphoglucose isomerase, Phosphohexose isomerase, Autocrine motility factor, Neuroleukin, Sperm antigen 36, GPI, PGI, PHI, AMF, NLK, SA-36, GNPI. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GPI solution (1mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ |
Glucose-6-phosphate isomerase (GPI) is a part of the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. GPI is a dimeric enzyme which catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI also functions as a tumor-secreted cytokine and an angiogenic factor (AMF) which stimulates endothelial cell motility. In addition, GPI is a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. GPI performs in different capacities inside and outside the cell. In the cytoplasm, GPI is involved in glycolysis and gluconeogenesis, while outside the cell it acts as a neurotrophic factor for spinal and sensory neurons. Defects in the GPI gene cause the nonspherocytic hemolytic anemia and a severe enzyme deficiency can be linked to hydrops fetalis, immediate neonatal death and neurological impairment.
GPI Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 578 amino acids (1-558 a.a.) and having a molecular mass of 65.3kDa.The GPI is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. |
| UniProt Protein Details: | Protein type:Isomerase; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; EC 5.3.1.9; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cytokine; Carbohydrate Metabolism - pentose phosphate pathway; Apoptosis Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol Molecular Function:monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; glycolysis; glucose 6-phosphate metabolic process; glucose metabolic process; pathogenesis; negative regulation of caspase activity; humoral immune response; gluconeogenesis; learning and/or memory; hemostasis; carbohydrate metabolic process; negative regulation of neuron apoptosis; angiogenesis Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency |
| NCBI Summary: | This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| UniProt Code: | P06744 |
| NCBI GenInfo Identifier: | 17380385 |
| NCBI Gene ID: | 2821 |
| NCBI Accession: | P06744.4 |
| UniProt Secondary Accession: | P06744,Q9BRD3, Q9BSK5, Q9UHE6, B4DG39, |
| UniProt Related Accession: | P06744 |
| Molecular Weight: | 558 |
| NCBI Full Name: | Glucose-6-phosphate isomerase |
| NCBI Synonym Full Names: | glucose-6-phosphate isomerase |
| NCBI Official Symbol: | GPI�� |
| NCBI Official Synonym Symbols: | AMF; NLK; PGI; PHI; GNPI; SA36; SA-36�� |
| NCBI Protein Information: | glucose-6-phosphate isomerase; neuroleukin; oxoisomerase; sperm antigen 36; sperm antigen-36; phosphohexomutase; phosphosaccharomutase; phosphohexose isomerase; phosphoglucose isomerase; autocrine motility factor; hexosephosphate isomerase; glucose phosph |
| UniProt Protein Name: | Glucose-6-phosphate isomerase |
| UniProt Synonym Protein Names: | Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36 |
| Protein Family: | Glucose-6-phosphate isomerase |
| UniProt Gene Name: | GPI�� |
| UniProt Entry Name: | G6PI_HUMAN |
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