Human GPD2 Recombinant Protein (RPPB1732)
- SKU:
- RPPB1732
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P43304
- Research Area:
- Enzymes
Description
| Product Name: | Human GPD2 Recombinant Protein |
| Product Code: | RPPB1732 |
| Size: | 10µg |
| Species: | Human |
| Target: | GPD2 |
| Synonyms: | Glycerol-3-phosphate dehydrogenase mitochondrial, glycerol-3-phosphate dehydrogenase 2 (mitochondrial), GPDH-M, GPD-M, mtGPD, GPD2, GDH2, GPDM, mGPDH. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | GPD2 protein solution is supplied in 20mM Tris-HCl pH 8, 1mM EDTA and 50% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
GPD2 (Mitochondrial glycerol-3-phosphate dehydrogenase) is a Ca2+-sensitive, FAD-binding protein, which is located on the outer surface of the inner mitochondrial membrane. Two isoforms have been identified for mGPD: Isoform 1 is comprised of 727 a.a. residues, while isoform 2 lacks 126 a.a. residues of the N-terminus. GPD2 catalyses the oxidation of glycerol-3-phosphate to DHAP (dihydroxyacetone phosphate) with associated reduction of the enzyme-bound FAD. GPD2 is a testis-specific promoter of mitochondrial GPDH. GPD2 along with a cytosolic NAD-linked GPD forms the glycerol phosphate shuttle that uses the interconversion of G-3-P and DHAP to transfer reducing equivalents into mitochondria, which results in the reoxidation of NADH produced during glycolysis.GPD2 deficiency contributes to the impairment of glucose-stimulated INS discharge in a number of animal models of non-INS dependent diabetes mellitus. GPD2 up-regulation as a result of a highly glycolytic environment contributes to the general increase in ROS generation and may lead to the progression of prostate cancer.
GPD2 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 558 amino acids fragment (43-600) corresponding to the GlpA domain fragment of the mature protein, having a total molecular mass of 66.26kDa and fused with a 4.5kDa amino-terminal hexahistidine tag. The GPD2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GPD2: localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010] |
| UniProt Protein Details: | Protein type:Lipid Metabolism - glycerophospholipid; EC 1.1.5.3; Mitochondrial; Oxidoreductase; Calcium-binding Chromosomal Location of Human Ortholog: 2q24.1 Cellular Component: mitochondrial inner membrane Molecular Function:glycerol-3-phosphate dehydrogenase activity Biological Process: cellular lipid metabolic process Disease: Diabetes Mellitus, Noninsulin-dependent |
| NCBI Summary: | The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010] |
| UniProt Code: | P43304 |
| NCBI GenInfo Identifier: | 229462943 |
| NCBI Gene ID: | 2820 |
| NCBI Accession: | P43304.3 |
| UniProt Secondary Accession: | P43304,Q59FR1, Q9HAP9, A8K4V0, B3KSA9, |
| UniProt Related Accession: | P43304 |
| Molecular Weight: | 67,531 Da |
| NCBI Full Name: | Glycerol-3-phosphate dehydrogenase, mitochondrial |
| NCBI Synonym Full Names: | glycerol-3-phosphate dehydrogenase 2 |
| NCBI Official Symbol: | GPD2�� |
| NCBI Official Synonym Symbols: | GDH2; GPDM; mGPDH�� |
| NCBI Protein Information: | glycerol-3-phosphate dehydrogenase, mitochondrial |
| UniProt Protein Name: | Glycerol-3-phosphate dehydrogenase, mitochondrial |
| UniProt Synonym Protein Names: | mtGPD |
| Protein Family: | Glycerol-3-phosphate dehydrogenase |
| UniProt Gene Name: | GPD2�� |
| UniProt Entry Name: | GPDM_HUMAN |
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