The Human GPC4 (Glypican 4) CLIA Kit is specifically designed for the quantitative measurement of Glypican 4 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reliable results for a variety of research applications.Glypican 4 is a critical member of the glypican family, known for its role in regulating cell growth, differentiation, and signaling pathways. Dysregulation of Glypican 4 has been implicated in various diseases, including cancer, developmental disorders, and cardiovascular conditions.
Therefore, measuring Glypican 4 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic interventions.The Human GPC4 CLIA Kit offered by AssayGenie provides researchers with a reliable tool for studying Glypican 4 and its implications in health and disease. With its high performance and user-friendly format, this kit is an essential resource for advancing research in the field of Glypican biology.
Product Name:
Human GPC4 (Glypican 4) CLIA Kit
SKU:
HUES00958
Target:
Human GPC4 (Glypican 4)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GPC4. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GPC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GPC4, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GPC4. You can calculate the concentration of Human GPC4 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
94-110
83-97
Average (%)
106
101
90
1:4
Range (%)
87-102
101-118
100-114
Average (%)
94
109
107
1:8
Range (%)
101-117
85-98
85-99
Average (%)
107
90
92
1:16
Range (%)
97-112
100-114
88-100
Average (%)
105
107
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
96
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
91-107
98
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.46
200.33
888.7
94.98
188.87
832.81
Standard deviation
10.5
19.75
101.93
10.54
17.98
71.21
C V (%)
10.15
9.86
11.47
11.1
9.52
8.55
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GPC4 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GPC4 in samples. No significant cross-reactivity or interference between Human GPC4 and analogues was observed.
