Human GP4/CD36 (Platelet Membrane Glycoprotein IV) ELISA Kit
The Human GP4/CD36 (Platelet Membrane Glycoprotein IV) ELISA Kit is a reliable and accurate tool for detecting levels of GP4/CD36 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.GP4/CD36 is a glycoprotein found on platelet membranes, playing a crucial role in platelet aggregation and thrombosis. Dysregulation of GP4/CD36 has been linked to various cardiovascular diseases and conditions such as atherosclerosis and stroke, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
With the Human GP4/CD36 ELISA Kit, researchers can accurately measure GP4/CD36 levels in human samples, providing valuable insights into platelet function and its implications in cardiovascular health. This kit is a valuable tool for research in the field of cardiovascular diseases and platelet biology.
Product Name:
Human GP4/CD36 (Platelet Membrane Glycoprotein IV) ELISA Kit
SKU:
HUES02146
Target:
Human GP4/CD36 (Platelet Membrane Glycoprotein IV)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GP4/CD36. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GP4/CD36 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GP4/CD36, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GP4/CD36. You can calculate the concentration of Human GP4/CD36 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-106
91-105
95-109
Average (%)
101
99
100
1:4
Range (%)
88-99
84-97
87-99
Average (%)
94
89
94
1:8
Range (%)
93-104
87-98
90-103
Average (%)
99
92
95
1:16
Range (%)
86-100
85-99
84-95
Average (%)
93
92
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-101
93
EDTA plasma (n=5)
91-107
98
Cell culture media (n=5)
91-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.54
0.89
4.68
0.54
0.82
4.4
Standard deviation
0.03
0.04
0.17
0.03
0.04
0.18
C V (%)
5.56
4.49
3.63
5.56
4.88
4.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GP4/CD36 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GP4/CD36 in samples. No significant cross-reactivity or interference between Human GP4/CD36 and analogues was observed.
