The Human GP39 (Glycoprotein-39) Cartilage CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of GP39 levels in human serum, plasma, and cell culture supernatants. With its superior sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for a diverse range of research endeavors.GP39, also known as chondromodulin-1, is a key glycoprotein found in cartilage that plays a crucial role in regulating cartilage growth and maintenance.
It has been implicated in various musculoskeletal disorders, making it a valuable marker for investigating these conditions and developing potential treatment strategies.Whether studying osteoarthritis, rheumatoid arthritis, or other cartilage-related pathologies, the Human GP39 Cartilage CLIA Kit provides researchers with the tools needed to unlock new insights into these complex diseases and advance the field of cartilage biology.
Product Name:
Human GP39 (Glycoprotein 39, Cartilage) CLIA Kit
SKU:
HUES00036
Target:
Human GP39 (Glycoprotein 39, Cartilage)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GP39. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GP39 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GP39, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GP39. You can calculate the concentration of Human GP39 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-110
95-110
92-107
Average (%)
103
102
98
1:4
Range (%)
88-104
97-112
100-117
Average (%)
95
103
109
1:8
Range (%)
100-117
92-107
92-105
Average (%)
107
100
98
1:16
Range (%)
90-106
95-111
96-108
Average (%)
97
103
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-109
104
EDTA plasma (n=5)
96-111
104
Cell culture media (n=5)
102-118
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
54.52
114.96
480.19
54.38
104.66
471.17
Standard deviation
6.48
9.1
36.16
6.65
11.62
47.54
C V (%)
11.89
7.92
7.53
12.23
11.1
10.09
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GP39 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GP39 in samples. No significant cross-reactivity or interference between Human GP39 and analogues was observed.
