Human GNMT Recombinant Protein (RPPB1718)
- SKU:
- RPPB1718
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q14749
- Research Area:
- Enzymes
Description
| Product Name: | Human GNMT Recombinant Protein |
| Product Code: | RPPB1718 |
| Size: | 10µg |
| Species: | Human |
| Target: | GNMT |
| Synonyms: | Glycine N-methyltransferase, GNMT, Glycine N-Methyltransferase, EC 2.1.1.20Epididymis Secretory Sperm Binding Protein Li 182mP, HEL-S-182mP. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | GNMT protein solution (1mg/ml) containing 20 mM Tris-HCl buffer (pH 8.0) and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MVDSVYRTRS LGVAAEGLPD QYADGEAARV WQLYIGDTRS RTAEYKAWLL GLLRQHGCQR VLDVACGTGV DSIMLVEEGF SVTSVDASDK MLKYALKERW NRRHEPAFDK WVIEEANWMT LDKDVPQSAE GGFDAVICLG NSFAHLPDCK GDQSEHRLAL KNIASMVRAG GLLVIDHRNY DHILSTGCAP PGKNIYYKSD LTKDVTTSVL IVNNKAHMVT LDYTVQVPGA GQDGSPGLSK FRLSYYPHCL ASFTELLQAA FGGKCQHSVL GDFKPYKPGQ TYIPCYFIHV LKRTD |
| Biological Activity: | Specific activity is > 100 nmol/min/mg, and is defined as the amount of enzyme that transfer 1.0 nmole of methyl group per minute at 37C. |
GNMT is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine with glycine to S-adenosyl-L-homocysteine and sarcosine. GNMT is located in the cytoplasm and acts as a homotetramer. Defects in the GNMT gene causes of GNMT deficiency (hypermethioninemia). GNMT affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and is involved in the detoxification pathway in liver cells. GNMT expression is diminished in human hepatocellular carcinoma (HCC). GNMT catalyzes the methylation of glycine by using s- adenosylmethionine (adomet) to form n-methylglycine (sarcosine) with the concomitant production of s-adenosylhomocysteine (adohcy). GNMT plays an essential role in the regulation of tissue concentration of adomet and of metabolism of methionine.
GNMT Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (1-295 a.a) and having a molecular mass of 34.9kDa.GNMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GNMT: Catalyzes the methylation of glycine by using S- adenosylmethionine (AdoMet) to form N-methylglycine (sarcosine) with the concomitant production of S-adenosylhomocysteine (AdoHcy). Possible crucial role in the regulation of tissue concentration of AdoMet and of metabolism of methionine. Defects in GNMT are the cause of glycine N- methyltransferase deficiency (GNMT deficiency); also known as hypermethioninemia. The only clinical abnormalities in patients with this deficiency are mild hepatomegaly and chronic elevation of serum transaminases. Belongs to the class I-like SAM-binding methyltransferase superfamily. Glycine N-methyltransferase family. |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - glycine, serine and threonine; Methyltransferase; EC 2.1.1.20 Chromosomal Location of Human Ortholog: 6p12 Cellular Component: cytoplasm Molecular Function:protein binding; glycine N-methyltransferase activity; glycine binding; folic acid binding Biological Process: methylation; glycogen metabolic process; S-adenosylmethionine metabolic process; protein modification process; regulation of gluconeogenesis; methionine metabolic process; one-carbon compound metabolic process; protein homotetramerization Disease: Glycine N-methyltransferase Deficiency |
| NCBI Summary: | The protein encoded by this gene is an enzyme that catalyzes the conversion of S-adenosyl-L-methionine (along with glycine) to S-adenosyl-L-homocysteine and sarcosine. The encoded protein is found in the cytoplasm and acts as a homotetramer. Defects in this gene are a cause of GNMT deficiency (hypermethioninemia). [provided by RefSeq, Oct 2008] |
| UniProt Code: | Q14749 |
| NCBI GenInfo Identifier: | 12644416 |
| NCBI Gene ID: | 27232 |
| NCBI Accession: | Q14749.3 |
| UniProt Secondary Accession: | Q14749,Q5T8W2, Q9NNZ1, Q9NS24, |
| UniProt Related Accession: | Q14749 |
| Molecular Weight: | 295 |
| NCBI Full Name: | Glycine N-methyltransferase |
| NCBI Synonym Full Names: | glycine N-methyltransferase |
| NCBI Official Symbol: | GNMT�� |
| NCBI Protein Information: | glycine N-methyltransferase; HEL-S-182mP; epididymis secretory sperm binding protein Li 182mP |
| UniProt Protein Name: | Glycine N-methyltransferase |
| Protein Family: | Glycine N-methyltransferase |
| UniProt Gene Name: | GNMT�� |
| UniProt Entry Name: | GNMT_HUMAN |
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