The Human GNLY (Granulysin) CLIA Kit is a cutting-edge assay designed for the precise measurement of granulysin levels in human samples. This kit offers exceptional sensitivity and specificity, guaranteeing consistent and accurate results for a variety of research purposes.Granulysin is a key protein involved in immune response, particularly in the destruction of target cells such as cancer cells and pathogens.
Its role in the immune system makes it a valuable biomarker for studying autoimmune diseases, infectious diseases, and immune-related disorders.This advanced CLIA kit from AssayGenie provides researchers with a powerful tool for investigating the role of granulysin in health and disease, offering insights that could lead to the development of innovative therapeutic strategies.
Product Name:
Human GNLY (Granulysin) CLIA Kit
SKU:
HUES00919
Target:
Human GNLY (Granulysin)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GNLY. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GNLY and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GNLY, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GNLY. You can calculate the concentration of Human GNLY in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
104-116
101-118
97-114
Average (%)
110
107
104
1:4
Range (%)
99-113
93-109
96-109
Average (%)
105
100
103
1:8
Range (%)
85-100
94-110
101-115
Average (%)
91
100
109
1:16
Range (%)
85-95
98-114
86-99
Average (%)
90
105
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
101-114
107
EDTA plasma (n=5)
90-104
96
Cell culture media (n=5)
85-101
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
194.19
573.95
1676.91
189.7
516.94
1813.74
Standard deviation
18.99
59.35
153.44
16.83
45.85
152.72
C V (%)
9.78
10.34
9.15
8.87
8.87
8.42
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GNLY concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GNLY in samples. No significant cross-reactivity or interference between Human GNLY and analogues was observed.
