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Human GNB3 Recombinant Protein (RPPB3619)

SKU:
RPPB3619
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P16520
€469

Description

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Product Name:Human GNB3 Recombinant Protein
Product Code:RPPB3619
Size:20µg
Species:Human
Target:GNB3
Synonyms:Transducin Beta Chain 3, G Protein, Beta-3 Subunit, GTP-BindingRegulatory Protein Beta-3 Chain, Guanine Nucleotide-Binding ProteinG(I)/G(S)/G(T) Beta Subunit 3, Guanine Nucleotide-Binding ProteinG(I)/G(S)/G(T) Subunit Beta-3, Hypertension Associated Protein, GuanineNucleotide Binding Protein (G Protein), Beta Polypeptide 3, GNB3.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:GNB3 protein solution (0.5mg/ml) containing 20mM Tris-HClbuffer (pH8.0), 10% glycerol and 0.4M Urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMGEMEQL RQEAEQLKKQ IADARKACAD VTLAELVSGL EVVGRVQMRT RRTLRGHLAK IYAMHWATDS KLLVSASQDG KLIVWDSYTT NKVHAIPLRS SWVMTCAYAP SGNFVACGGL DNMCSIYNLK SREGNVKVSR ELSAHTGYLS CCRFLDDNNI VTSSGDTTCA LWDIETGQQK TVFVGHTGDC MSLAVSPDFN LFISGACDAS AKLWDVREGT CRQTFTGHES DINAICFFPN GEAICTGSDD ASCRLFDLRA DQELICFSHE SIICGITSVA FSLSGRLLFA GYDDFNCNVW DSMKSERVGI LSGHDNRVSC LGVTADGMAV ATGSWDSFLK IWN

GNB3belongs to the WD repeat G protein beta family. GNB3 is an important regulatorof alpha subunit, as well as of certain signal transduction receptor and effector.A single-nucleotide polymorphism (C825T) in GNB3 is linked with essentialhypertension and obesity. In addition, this polymorphism is also linked withthe occurrence of the splice variant GNB3-s, which seems to have increasedactivity. GNB3-s is an example of alternative splicing caused by a nucleotidechange outside of the splice donor and acceptor sites. Further splice variantsmay exist for GNB3, however they have not been fully described. Among thediseases associated with GNB3 are syncope, and aortic coarctation.

GNB3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 363 amino acids (1-340 a.a) and having a molecular mass of 39.6kDa.GNB3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:G-beta 3: Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein- effector interaction. G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2. Belongs to the WD repeat G protein beta family.
UniProt Protein Details:

Protein type:G protein; G protein, heterotrimeric beta; G protein, heterotrimeric

Chromosomal Location of Human Ortholog: 12p13

Cellular Component: neuron projection; plasma membrane

Molecular Function:GTPase activity; protein binding; GTPase binding; signal transducer activity; spectrin binding

Biological Process: synaptic transmission; G-protein coupled receptor protein signaling pathway; regulation of blood pressure; energy reserve metabolic process

Disease: Hypertension, Essential

NCBI Summary:Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit. These subunits are encoded by families of related genes. This gene encodes a beta subunit which belongs to the WD repeat G protein beta family. Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors. A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity. This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity. GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2014]
UniProt Code:P16520
NCBI GenInfo Identifier:121011
NCBI Gene ID:2784
NCBI Accession:P16520.1
UniProt Related Accession:P16520
Molecular Weight:37kDa
NCBI Full Name:Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
NCBI Synonym Full Names:G protein subunit beta 3
NCBI Official Symbol:GNB3��
NCBI Official Synonym Symbols:CSNB1H��
NCBI Protein Information:guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
UniProt Protein Name:Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
UniProt Synonym Protein Names:Transducin beta chain 3
UniProt Gene Name:GNB3��
UniProt Entry Name:GBB3_HUMAN

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