Human GMPS Recombinant Protein (RPPB1716)
- SKU:
- RPPB1716
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P49915
- Research Area:
- Enzymes
Description
| Product Name: | Human GMPS Recombinant Protein |
| Product Code: | RPPB1716 |
| Size: | 10µg |
| Species: | Human |
| Target: | GMPS |
| Synonyms: | GMP synthase [glutamine-hydrolyzing], GMP synthetase, Glutamine amidotransferase, GMPS, GMP synthase, guanosine 5'-monophosphate synthase, MLL/GMPS fusion protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GMPS solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 30% glycerol and 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMALCNG DSKLENAGGD LKDGHHHYEG AVVILDAGAQ YGKVIDRRVR ELFVQSEIFP LETPAFAIKE QGFRAIIISG GPNSVYAEDA PWFDPAIFTI GKPVLGICYG MQMMNKVFGG TVHKKSVRED GVFNISVDNT CSLFRGLQKE EVVLLTHGDS VDKVADGFKV VARSGNIVAG IANESKKLYG AQFHPEVGLT ENGKVILKNF LYDIAGCSGT FTVQNRELEC IREIKERVGT SKVLVLLSGG VDSTVCTALL NRALNQEQVI AVHIDNGFMR KRESQSVEEA LKKLGIQVKV INAAHSFYNG TTTLPISDED RTPRKRISKT LNMTTSPEEK RKIIGDTFVK IANEVIGEMN LKPEEVFLAQ GTLRPDLIES ASLVASGKAE LIKTHHNDTE LIRKLREEGK VIEPLKDFHK DEVRILGREL GLPEELVSRH PFPGPGLAIR VICAEEPYIC KDFPETNNIL KIVADFSASV KKPHTLLQRV KACTTEEDQE KLMQITSLHS LNAFLLPIKT VGVQGDCRSY SYVCGISSKD EPDWESLIFL ARLIPRMCHN VNRVVYIFGP PVKEPPTDVT PTFLTTGVLS TLRQADFEAH NILRESGYAG KISQMPVILT PLHFDRDPLQ KQPSCQRSVV IRTFITSDFM TGIPATPGNE IPVEVVLKMV TEIKKIPGIS RIMYDLTSKP PGTTEWE |
GMP synthase (GMPS) is involved in purine biosynthesis. GMPS, which is a homodimer, catalyzes the last step in the GMP synthesis pathway, specifically the ATP-dependent amination of XMP to GMP. GMPS is comprised of one GMP-binding domain and one glutamine amidotransferase type-1 domain through which it communicates its catalytic activity. GMPS is engaged in the de novo synthesis of nucleotides which are not only vital for DNA and RNA synthesis, but also supply GTP, which is involved in sevral cellular processes important for cell division. GMPS gene chromosomal translocations are linked with acute myeloid leukemias, suggesting a possible role for GMPS in carcinogenesis.
GMPS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 717 amino acids (1-693) and having a molecular mass of 79.2kDa.GMPS is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GMPS: Involved in the de novo synthesis of guanine nucleotides which are not only essential for DNA and RNA synthesis, but also provide GTP, which is involved in a number of cellular processes important for cell division. A chromosomal aberration involving GMPS is found in acute myeloid leukemias. Translocation t(3,11)(q25,q23) with MLL. |
| UniProt Protein Details: | Protein type:Oncoprotein; Nucleotide Metabolism - purine; Xenobiotic Metabolism - drug metabolism - other enzymes; Ligase; EC 6.3.5.2 Chromosomal Location of Human Ortholog: 3q24 Cellular Component: cytoplasm; cytosol Molecular Function:GMP synthase (glutamine-hydrolyzing) activity; GMP synthase activity Biological Process: purine base biosynthetic process; purine ribonucleoside monophosphate biosynthetic process Disease: Leukemia, Acute Myeloid |
| NCBI Summary: | In the de novo synthesis of purine nucleotides, IMP is the branch point metabolite at which point the pathway diverges to the synthesis of either guanine or adenine nucleotides. In the guanine nucleotide pathway, there are 2 enzymes involved in converting IMP to GMP, namely IMP dehydrogenase (IMPD1), which catalyzes the oxidation of IMP to XMP, and GMP synthetase, which catalyzes the amination of XMP to GMP. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P49915 |
| NCBI GenInfo Identifier: | 1708072 |
| NCBI Gene ID: | 8833 |
| NCBI Accession: | P49915.1 |
| UniProt Secondary Accession: | P49915,A8K639, B4DXV7, F8W720, |
| UniProt Related Accession: | P49915 |
| Molecular Weight: | 65,929 Da |
| NCBI Full Name: | GMP synthase |
| NCBI Synonym Full Names: | guanine monophosphate synthase |
| NCBI Official Symbol: | GMPS�� |
| NCBI Protein Information: | GMP synthase [glutamine-hydrolyzing] |
| UniProt Protein Name: | GMP synthase [glutamine-hydrolyzing] |
| UniProt Synonym Protein Names: | GMP synthetase; Glutamine amidotransferase |
| UniProt Gene Name: | GMPS�� |
| UniProt Entry Name: | GUAA_HUMAN |
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