Human GMDS Recombinant Protein (RPPB1713)
- SKU:
- RPPB1713
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O60547
- Research Area:
- Enzymes
Description
| Product Name: | Human GMDS Recombinant Protein |
| Product Code: | RPPB1713 |
| Size: | 20µg |
| Species: | Human |
| Target: | GMDS |
| Synonyms: | GDP-mannose 4,6-dehydratase, GMD, SDR3E1, short chain dehydrogenase/reductase family 3E member 1, GDP-D-mannose dehydratase, EC 4.2.1.47. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GMDS solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl, 0.1mM PMSF and 30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAHAPARCPS ARGSGDGEMG KPRNVALITG ITGQDGSYLA EFLLEKGYEV HGIVRRSSSF NTGRIEHLYK NPQAHIEGNM KLHYGDLTDS TCLVKIINEV KPTEIYNLGA QSHVKISFDL AEYTADVDGV GTLRLLDAVK TCGLINSVKF YQASTSELYG KVQEIPQKET TPFYPRSPYG AAKLYAYWIV VNFREAYNLF AVNGILFNHE SPRRGANFVT RKISRSVAKI YLGQLECFSL GNLDAKRDWG HAKDYVEAMW LMLQNDEPED FVIATGEVHS VREFVEKSFL HIGKTIVWEG KNENEVGRCK ETGKVHVTVD LKYYRPTEVD FLQGDCTKAK QKLNWKPRVA FDELVREMVH ADVELMRTNP NA |
GMDS is a member of the GDP-mannose 4,6-dehydratase family. GMDS uses NADP as a cofactor to catalyze the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose. Defects in the gene encoding GMDS cause TRAIL (tumor necrosis factor-related apoptosis-inducing ligand)-induced apoptosis.
GMDS Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 392 amino acids (1-372) and having a molecular mass of 44.1 kDa.GMDS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GMD: an enzyme that converts GDP-D-mannose to GDP-4-keto-6-D- deoxymannose. Perticipates in the extravasation of activated lymphocytes. |
| UniProt Protein Details: | Protein type:Lyase; Carbohydrate Metabolism - fructose and mannose; EC 4.2.1.47; Carbohydrate Metabolism - amino sugar and nucleotide sugar Chromosomal Location of Human Ortholog: 6p25 Cellular Component: cytoplasm Molecular Function:GDP-mannose 4,6-dehydratase activity Biological Process: Notch signaling pathway; GDP-mannose metabolic process; 'de novo' GDP-L-fucose biosynthetic process |
| NCBI Summary: | GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).[supplied by OMIM, Aug 2009] |
| UniProt Code: | O60547 |
| NCBI GenInfo Identifier: | 9087147 |
| NCBI Gene ID: | 2762 |
| NCBI Accession: | O60547.1 |
| UniProt Secondary Accession: | O60547,O75357, Q5T954, Q6FH09, Q9UGZ3, Q9UJK9, E9PI88 |
| UniProt Related Accession: | O60547 |
| Molecular Weight: | 372 |
| NCBI Full Name: | GDP-mannose 4,6 dehydratase |
| NCBI Synonym Full Names: | GDP-mannose 4,6-dehydratase |
| NCBI Official Symbol: | GMDS�� |
| NCBI Official Synonym Symbols: | GMD; SDR3E1�� |
| NCBI Protein Information: | GDP-mannose 4,6 dehydratase; GDP-D-mannose dehydratase; short chain dehydrogenase/reductase family 3E, member 1 |
| UniProt Protein Name: | GDP-mannose 4,6 dehydratase |
| UniProt Synonym Protein Names: | GDP-D-mannose dehydratase; GMD |
| Protein Family: | GDP-mannose 4,6 dehydratase |
| UniProt Gene Name: | GMDS�� |
| UniProt Entry Name: | GMDS_HUMAN |
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