Human GM CSF Recombinant Protein (RPPB0354)
- SKU:
- RPPB0354
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P04141
- Research Area:
- Growth Factors & Cytokines
Description
| Product Name: | Human GM CSF Recombinant Protein |
| Product Code: | RPPB0354 |
| Size: | 10µg |
| Species: | Human |
| Target: | GM CSF |
| Synonyms: | CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897. |
| Source: | Pichia Pastoris |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The protein was lyophilized from a concentrated (1mg/ml) solution containing 10mM phosphate buffer pH 7.0, 40 mg mannitol and 10 mg sucrose. |
| Solubility: | It is recommended to reconstitute the lyophilized Granulocyte Macrophage Colony Stimulating Factor in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution GMCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE. |
| Amino Acid Sequence: | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser |
| Biological Activity: | The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is < 0.183 ng/ml, corresponding to a Specific Activity of 5,500,000IU/mg. |
GMCSF is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in Yeast is a single, glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 26-32 kDa. rhGMCSF differs from the natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. GM-CSF is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GMCSF: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Belongs to the GM-CSF family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted; Cytokine Chromosomal Location of Human Ortholog: 5q31.1 Cellular Component: extracellular space; extracellular region Molecular Function:protein binding; growth factor activity; granulocyte macrophage colony-stimulating factor receptor binding; cytokine activity Biological Process: negative regulation of cytolysis; epithelial fluid transport; embryonic placenta development; macrophage activation; positive regulation of tyrosine phosphorylation of Stat5 protein; positive regulation of interleukin-23 production; positive regulation of cell proliferation; myeloid dendritic cell differentiation; immune response; positive regulation of DNA replication |
| NCBI Summary: | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P04141 |
| NCBI GenInfo Identifier: | 117561 |
| NCBI Gene ID: | 1437 |
| NCBI Accession: | P04141.1 |
| UniProt Secondary Accession: | P04141,Q14CE8, Q2VPI8, Q8NFI6, |
| UniProt Related Accession: | P04141 |
| Molecular Weight: | 16,295 Da |
| NCBI Full Name: | Granulocyte-macrophage colony-stimulating factor |
| NCBI Synonym Full Names: | colony stimulating factor 2 (granulocyte-macrophage) |
| NCBI Official Symbol: | CSF2�� |
| NCBI Official Synonym Symbols: | GMCSF�� |
| NCBI Protein Information: | granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim |
| UniProt Protein Name: | Granulocyte-macrophage colony-stimulating factor |
| UniProt Synonym Protein Names: | Colony-stimulating factor; CSF; Molgramostin; Sargramostim |
| Protein Family: | Granulocyte-macrophage colony-stimulating factor |
| UniProt Gene Name: | CSF2�� |
| UniProt Entry Name: | CSF2_HUMAN |
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