Human Glutathione S Transferase Theta 1 (GSTt1) ELISA Kit
The Human Glutathione S-Transferase Theta 1 (GSTT1) ELISA Kit is a powerful tool for accurately measuring levels of GSTT1 in human samples. This kit offers exceptional sensitivity and specificity, guaranteeing precise and consistent results for various research purposes.GSTT1 is an important enzyme involved in the detoxification of harmful substances in the body, playing a key role in protecting cells from oxidative damage. Dysregulation of GSTT1 has been linked to various health conditions, making it a valuable biomarker for studying diseases such as cancer, liver diseases, and drug metabolism.
With the Human GSTT1 ELISA Kit, researchers can explore the role of GSTT1 in disease pathology, drug responses, and environmental exposures, paving the way for potential therapeutic interventions. Trust in the accuracy and reliability of this kit for all your GSTT1 research needs.
Product Name:
Human Glutathione S Transferase Theta 1 (GSTt1) ELISA Kit
Product Code:
HUDL01280
Size:
96 Assays
Target:
GSTt1
Synonyms:
GST-T1
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
82-102
92
EDTA plasma (n=5)
91-98
94
Heparin plasma (n=5)
86-101
93
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
