Human Glutathione S Transferase Mu 1 (GSTm1) ELISA Kit
The Human Glutathione S-transferase mu 1 (GSTm1) ELISA Kit is a highly accurate and sensitive assay designed for the detection of GSTm1 levels in human samples such as serum, plasma, and cell culture supernatants. With its high specificity, this kit provides reliable and reproducible results, making it suitable for a wide range of research applications.GSTm1 is an important enzyme involved in the detoxification of various drugs, toxins, and carcinogens in the body. Its levels have been linked to the risk of developing certain diseases such as cancer, cardiovascular diseases, and neurodegenerative disorders.
Therefore, this ELISA kit is a valuable tool for studying these conditions and exploring potential therapeutic interventions.Overall, the Human GSTm1 ELISA Kit offers researchers a reliable method for quantifying GSTm1 levels in human samples, making it an essential component of any laboratory studying the role of GSTm1 in health and disease.
Product Name:
Human Glutathione S Transferase Mu 1 (GSTm1) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
88-100
94
EDTA plasma (n=5)
90-101
95
Heparin plasma (n=5)
92-101
96
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
