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Human Glutathione Peroxidase 4 (GPX4) ELISA Kit

SKU:
HUDL01248
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P36969
Sensitivity:
0.061ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MCSP,PHGPx,SnGPx,SnPHGPx,Phospholipid Hydroperoxidase,Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Glutathione Peroxidase 4 (GPX4) ELISA Kit

The Human Glutathione Peroxidase 4 (GPX4) ELISA Kit is a powerful tool for the accurate detection of GPX4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a wide range of research applications.GPX4 is a key enzyme involved in protecting cells from oxidative stress and lipid peroxidation. It plays a vital role in various health conditions, including cancer, neurodegenerative diseases, and aging.

Monitoring GPX4 levels can provide valuable insights into disease progression and potential therapeutic targets.With reliable performance and easy-to-use protocols, the Human GPX4 ELISA Kit is an essential tool for researchers studying oxidative stress, cell survival, and disease mechanisms. Invest in this kit to advance your research and uncover new insights into the role of GPX4 in human health and disease.

Product Name:Human Glutathione Peroxidase 4 (GPX4) ELISA Kit
Product Code:HUDL01248
Size:96 Assays
Target:GPX4
Synonyms:MCSP, PHGPx, SnGPx, SnPHGPx, Phospholipid Hydroperoxidase, Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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