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Human Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit

SKU:
HUDL01247
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P22352
Sensitivity:
2.42ng/mL
Range:
6.25-400ng/mL
ELISA Type:
Sandwich
Synonyms:
GSHPX3,GPx-P,GSHPx-P,GPXP,Plasma glutathione peroxidase,Extracellular glutathione peroxidase
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit

The Human Glutathione Peroxidase 3 (GPX3) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of GPX3 levels in human plasma samples. GPX3 is an important antioxidant enzyme that plays a key role in protecting cells from oxidative damage by reducing hydrogen peroxide and lipid hydroperoxides.This ELISA kit allows for precise and reliable measurement of GPX3 levels in a variety of research applications, providing valuable insights into the antioxidant defense system and its role in various diseases and conditions.

With its high sensitivity and specificity, this kit ensures reproducible results that can advance research in areas such as cancer, cardiovascular diseases, and aging.Overall, the Human GPX3 ELISA Kit is a valuable tool for studying the function of GPX3 in health and disease, offering researchers a reliable method for quantifying this important antioxidant enzyme in human plasma samples.

Product Name:Human Glutathione Peroxidase 3, Plasma (GPX3) ELISA Kit
Product Code:HUDL01247
Size:96 Assays
Target:GPX3
Synonyms:GSHPX3, GPx-P, GSHPx-P, GPXP, Plasma glutathione peroxidase, Extracellular glutathione peroxidase
Detection Method:Sandwich
Range:6.25-400ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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