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Human Glutaminyl Peptide Cyclotransferase (QPCT) ELISA Kit

SKU:
HUDL02480
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q16769
Sensitivity:
0.071ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
GCT,QC,EC,Glutaminyl Cyclase,Glutaminyl-tRNA cyclotransferase
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Glutaminyl Peptide Cyclotransferase (QPCT) ELISA Kit

The Human Glutaminyl Peptide Cyclotransferase (QPCT) ELISA Kit is a powerful tool for the precise measurement of QPCT levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it suitable for a wide range of research applications.QPCT is an important enzyme involved in peptide processing and is linked to various diseases, including neurodegenerative disorders and cardiovascular conditions.

By detecting and quantifying QPCT levels, researchers can gain valuable insights into the pathophysiology of these diseases and potentially identify new therapeutic targets.Order the Human Glutaminyl Peptide Cyclotransferase (QPCT) ELISA Kit today and enhance your research capabilities in the fields of neuroscience, cardiology, and beyond.

Product Name:Human Glutaminyl Peptide Cyclotransferase (QPCT) ELISA Kit
Product Code:HUDL02480
Size:96 Assays
Target:QPCT
Synonyms:GCT, QC, EC, Glutaminyl Cyclase, Glutaminyl-tRNA cyclotransferase
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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