Description
Product Name: | Human GAD IgG Autoantibodies ELISA Kit |
SKU: | HDES0076 |
Size: | 96T |
Target: | GAD-Ab |
Detection Method: | Indirect ELISA |
Reactivity: | Human |
Result Type: | Qualitative: Sensitivity 98.5%, Specificity 99.8% |
Sample Type: | Serum |
Detection Wavelength: | 450/630nm |
Storage: | The majority of kits have a shelf-life of 12 months from the production date at 2-8℃ |
This ELISA kit uses Indirect-ELISA as the method to detect the Glutamic Acid Decarboxylase (GAD) antibody (IgG) in human serum. The ELISA Microtiter plate provided in this kit has been pre-coated with purified GAD antigen. Samples are added to the ELISA Microtiter plate wells and the GAD-Ab in which will combine with the pre-coated antigen to form antigen-antibody compound. Free components are washed away. The HRP conjugate is added to each well and react with the compound to form “GAD antigen- GAD antibody-HRP conjugate” compound. The TMB substrate is added to initiate the color developing reaction. The presence of GAD- IgG can be determined according to the OD value by using a microplate reader with 450 nm (630 nm) wavelength.