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Human Glutamate Decarboxylase Like Protein 1 (GADL1) ELISA Kit

SKU:
HUDL01138
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q6ZQY3
Sensitivity:
0.083ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ADC,CSADC,Acidic amino acid decarboxylase GADL1,Aspartate 1-decarboxylase,Cysteine sulfinic acid decarboxylase
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€749
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Description

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Human Glutamate Decarboxylase Like Protein 1 (GADL1) ELISA Kit

The Human GADL1 (Glutamate Decarboxylase-Like Protein 1) ELISA Kit is specially designed for the accurate measurement of GADL1 levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers unmatched sensitivity and specificity, ensuring precise and consistent results that are essential for various research applications.GADL1 is a critical enzyme that plays a role in the metabolism of glutamate, a key neurotransmitter in the brain.

Dysregulation of GADL1 has been associated with neurological disorders such as epilepsy and schizophrenia, highlighting its importance as a potential biomarker for studying these conditions and developing targeted therapies.With its reliable performance and ease of use, the Human GADL1 ELISA Kit is a valuable tool for researchers focused on understanding the role of GADL1 in health and disease.

Product Name:Human Glutamate Decarboxylase Like Protein 1 (GADL1) ELISA Kit
Product Code:HUDL01138
Size:96 Assays
Target:GADL1
Synonyms:ADC, CSADC, Acidic amino acid decarboxylase GADL1, Aspartate 1-decarboxylase, Cysteine sulfinic acid decarboxylase
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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