Human Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit
The Human Glutamate-Cysteine Ligase Modifier Subunit GCLM ELISA Kit is a powerful tool specifically designed for the accurate quantification of GCLM levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides reliable and reproducible results, making it an invaluable resource for various research applications.GCLM is a key component of the glutamate-cysteine ligase enzyme, which is essential for the synthesis of glutathione, a crucial antioxidant involved in cellular protection against oxidative stress. Dysregulation of GCLM expression has been linked to various diseases, including cancer, neurodegenerative disorders, and metabolic syndromes, highlighting its significance as a potential therapeutic target and biomarker for disease progression.
By utilizing the Human Glutamate-Cysteine Ligase Modifier Subunit GCLM ELISA Kit, researchers can gain insights into the mechanisms underlying oxidative stress-related diseases and explore novel therapeutic strategies aimed at restoring cellular redox balance. This kit is an essential tool for advancing scientific knowledge and driving innovation in the field of biomedicine.
Product Name:
Human Glutamate Cysteine Ligase, Modifier Subunit (GCLM) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
