The Human Glut3 (Glucose Transporter 3) CLIA Kit is a highly reliable and sensitive assay designed for the accurate quantification of Glut3 levels in human samples such as serum, plasma, and cell culture supernatants. This kit provides researchers with a robust tool to study the role of Glut3 in glucose transport and metabolism.Glut3 is a key glucose transporter protein that plays a crucial role in facilitating glucose uptake in various tissues and cells. Dysregulation of Glut3 expression has been implicated in metabolic disorders such as diabetes and cancer, highlighting its importance as a potential therapeutic target.
With its high specificity and reproducibility, the Human Glut3 CLIA Kit offers researchers a valuable tool for investigating the role of Glut3 in various physiological and pathological conditions. Whether studying glucose metabolism, metabolic disorders, or cancer biology, this kit provides accurate and reliable results for a wide range of research applications.
Product Name:
Human GLUT3 (Glucose Transporter 3) CLIA Kit
SKU:
HUES01010
Target:
Human GLUT3 (Glucose Transporter 3)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GLUT3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLUT3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLUT3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GLUT3. You can calculate the concentration of Human GLUT3 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
92-107
94-109
Average (%)
106
98
99
1:4
Range (%)
89-102
101-117
98-112
Average (%)
96
109
106
1:8
Range (%)
83-98
97-112
96-109
Average (%)
90
104
102
1:16
Range (%)
99-111
86-101
99-115
Average (%)
104
93
106
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-106
96
EDTA plasma (n=5)
87-98
92
Cell culture media (n=5)
86-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.02
219.27
690.07
90.57
198.36
747.6
Standard deviation
9.94
25.7
53.83
10.02
16.38
77.53
C V (%)
10.57
11.72
7.8
11.06
8.26
10.37
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GLUT3 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLUT3 in samples. No significant cross-reactivity or interference between Human GLUT3 and analogues was observed.
