The Human GLUT1 (Glucose Transporter 1) CLIA Kit is specifically designed for the precise measurement of GLUT1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and consistent results, making it an ideal tool for various research applications.GLUT1 is a key protein responsible for glucose transport across cell membranes, playing a crucial role in cellular glucose uptake and metabolism. Dysregulation of GLUT1 expression has been associated with various health conditions, including diabetes, cancer, and neurological disorders, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.
This GLUT1 CLIA Kit from Assay Genie offers researchers a reliable and efficient method for quantifying GLUT1 levels, enabling a better understanding of glucose metabolism and its implications in disease pathology. Whether investigating metabolic disorders or exploring new treatment approaches, this kit provides a valuable tool for advancing research in the field of cellular glucose transport.
Product Name:
Human GLUT1 (Glucose Transporter 1) CLIA Kit
SKU:
HUES01009
Target:
Human GLUT1 (Glucose Transporter 1)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
37.50 pg/mL
Detection range:
62.50-4000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GLUT1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLUT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLUT1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GLUT1. You can calculate the concentration of Human GLUT1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
94-106
94-106
95-112
Average (%)
99
100
102
1:4
Range (%)
93-106
99-115
86-98
Average (%)
100
106
92
1:8
Range (%)
93-105
100-116
95-108
Average (%)
100
107
101
1:16
Range (%)
86-100
98-114
85-98
Average (%)
93
104
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-105
97
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
204.74
537.59
1383.29
197.46
513.78
1487.62
Standard deviation
21.33
52.31
122.42
18.56
50.4
122.73
C V (%)
10.42
9.73
8.85
9.4
9.81
8.25
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GLUT1 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLUT1 in samples. No significant cross-reactivity or interference between Human GLUT1 and analogues was observed.
