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Human Glucagon Like Peptide 1 (GLP1) ELISA Kit

SKU:
HUDL01200
Product Type:
ELISA Kit
Sensitivity:
5.44pg/mL
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human Glucagon Like Peptide 1 (GLP1) ELISA Kit

The Human Glucagon-Like Peptide 1 (GLP-1) ELISA Kit is designed for the accurate measurement of GLP-1 levels in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications. GLP-1 is a hormone involved in regulating glucose levels and satiety, making it a key player in diabetes and obesity research.

Monitoring GLP-1 levels can provide valuable insights into metabolic disorders and potential treatment options. The Human GLP-1 ELISA Kit is a valuable tool for researchers studying these conditions and exploring novel therapies.

Product Name:Human Glucagon Like Peptide 1 (GLP1) ELISA Kit
Product Code:HUDL01200
Size:96 Assays
Target:GLP1
Detection Method:Competition
Range:15.6-1,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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