The Glucagon-Like Peptide-1 (GLP-1) ELISA Kit is specially designed for the accurate quantitative measurement of GLP-1 levels in human samples. GLP-1, a critical gut hormone, plays a key role in regulating glucose homeostasis and insulin release, making it a vital component of blood sugar control and metabolism. Monitoring GLP-1 levels is essential for understanding metabolic processes, particularly in conditions such as diabetes and metabolic disorders. This ELISA kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for the quantification of GLP-1 in various biological samples.
Manufactured under stringent quality control standards, the GLP-1 ELISA Kit provides reliable performance and user-friendly protocols, making it suitable for both research and clinical applications. Trust in the GLP-1 ELISA Kit from Assay Genie for accurate and dependable quantification of this essential biomarker in your scientific investigations.
Product Name:
Human GLP-1 (Glucagon Like Peptide 1) ELISA Kit
SKU:
AEES00061
Target:
Human GLP-1 (Glucagon Like Peptide 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.94 pg/mL
Detection range:
1.56-100 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human GLP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GLP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GLP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human GLP-1. You can calculate the concentration of Human GLP-1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-100
87-98
85-97
Average (%)
92
92
93
1:4
Range (%)
87-98
93-104
86-97
Average (%)
95
101
92
1:8
Range (%)
98-112
95-101
88-98
Average (%)
104
98
93
1:16
Range (%)
93-108
84-97
94-102
Average (%)
101
92
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-104
101
EDTA plasma (n=5)
100-108
103
Cell culture media (n=5)
94-106
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
4.59
12.02
49.67
4.23
11.18
42.57
Standard deviation
0.25
0.59
2.52
0.23
0.51
1.89
C V (%)
5.45
4.91
5.07
5.44
4.56
4.44
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human GLP-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GLP-1 in samples. No significant cross-reactivity or interference between Human GLP-1 and analogues was observed.
