Human GLO1 Recombinant Protein (RPPB1695)
- SKU:
- RPPB1695
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q04760
- Research Area:
- Enzymes
Description
| Product Name: | Human GLO1 Recombinant Protein |
| Product Code: | RPPB1695 |
| Size: | 10µg |
| Species: | Human |
| Target: | GLO1 |
| Synonyms: | GLYI, GLOD1, GLO1, Glyoxalase-1, Lactoylglutathione lyase, Methylglyoxalase, Aldoketomutase, Ketone-aldehyde mutase, Glyoxalase I, S-D-lactoylglutathione methylglyoxal lyase, Glx I. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | Glyoxalase-1 solution containing 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIAWALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM |
| Biological Activity: | Specificactivity: > 400 units/mg. One unit will form 1.0umol of S-lactoylgutathionefrom methylglyoxal and reduced glutathione per minute at pH6.5 at 25C |
GLO1 is involved in the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. GLO1 is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. GLO1 enzyme is ubundantly expressed and present in numerous tumor cell lines, in which its concentration is often upregulated ubiquitisly. GLO1 is a major susceptible gene for autism in an ethnic Chinese population from Taiwan. GLO1 might be involved in the pathophysiology of mood disorders. GLO1 plays a role in the pathophysiology of mood disorders. Overexpression of GLO1 is associated with kidney tumor.
Glyoxalase-I Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 184 amino acids and having a molecular mass of 20.7 kDa. Glyoxalase-1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GLO1: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF- kappa-B. Belongs to the glyoxalase I family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Carbohydrate Metabolism - pyruvate; EC 4.4.1.5; Lyase Chromosomal Location of Human Ortholog: 6p21.3-p21.1 Cellular Component: cytoplasm Molecular Function:zinc ion binding; lactoylglutathione lyase activity Biological Process: regulation of transcription from RNA polymerase II promoter; glutathione metabolic process; carbohydrate metabolic process; osteoclast differentiation; methylglyoxal metabolic process; negative regulation of apoptosis Disease: Autism |
| NCBI Summary: | The enzyme encoded by this gene is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. Glyoxalase I is linked to HLA and is localized to 6p21.3-p21.1, between HLA and the centromere. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q04760 |
| NCBI GenInfo Identifier: | 134039205 |
| NCBI Gene ID: | 2739 |
| NCBI Accession: | Q04760.4 |
| UniProt Secondary Accession: | Q04760,P78375, Q59EL0, Q5TZW3, Q96FC0, Q96J41, B2R6P7 B4DDV0, |
| UniProt Related Accession: | Q04760 |
| Molecular Weight: | Calculated: 19kDa/20kDaObserved: 21kDa |
| NCBI Full Name: | Lactoylglutathione lyase |
| NCBI Synonym Full Names: | glyoxalase I |
| NCBI Official Symbol: | GLO1�� |
| NCBI Official Synonym Symbols: | GLYI; GLOD1; HEL-S-74�� |
| NCBI Protein Information: | lactoylglutathione lyase; glx I; aldoketomutase; methylglyoxalase; ketone-aldehyde mutase; lactoyl glutathione lyase; glyoxalase domain containing 1; epididymis secretory protein Li 74; S-D-lactoylglutathione methylglyoxal lyase |
| UniProt Protein Name: | Lactoylglutathione lyase |
| UniProt Synonym Protein Names: | Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase |
| UniProt Gene Name: | GLO1�� |
| UniProt Entry Name: | LGUL_HUMAN |
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