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Human Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit

SKU:
HUDL01174
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P39905
Sensitivity:
0.053ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
ATF1,ATF2,HFB1-GDNF,Astrocyte-Derived Trophic Factor,Glial Derived Neurotrophic Factor
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cerebrospinal Fluid, Cell Culture Supernatants And Other Biological Fluids
€599
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Description

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Human Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit

The Human Glial Cell Line-Derived Neurotrophic Factor (GDNF) ELISA Kit is specifically designed for the precise quantification of GDNF levels in human samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for a variety of research purposes.GDNF is a vital neurotrophic factor that plays a crucial role in the maintenance and survival of neurons in the central and peripheral nervous systems.

Dysregulation of GDNF levels has been implicated in various neurodegenerative diseases such as Parkinson's and Alzheimer's, making it an important biomarker for studying these conditions and potentially developing therapeutic interventions.Overall, the Human GDNF ELISA Kit provides researchers with a valuable tool for investigating the role of GDNF in neurological disorders and advancing our understanding of neurotrophic factors in human health and disease.

Product Name:Human Glial Cell Line Derived Neurotrophic Factor (GDNF) ELISA Kit
Product Code:HUDL01174
Size:96 Assays
Target:GDNF
Synonyms:ATF1, ATF2, HFB1-GDNF, Astrocyte-Derived Trophic Factor, Glial Derived Neurotrophic Factor
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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