Human GINS4 Recombinant Protein (RPPB3594)
- SKU:
- RPPB3594
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9BRT9
Description
| Product Name: | Human GINS4 Recombinant Protein |
| Product Code: | RPPB3594 |
| Size: | 10µg |
| Species: | Human |
| Target: | GINS4 |
| Synonyms: | SLD5, GINS complex subunit 4, DNA replication complex GINS protein SLD5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | GINS4 protein solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl,20% glycerol and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MTEEVDFLGQ DSDGGSEEVV LTPAELIERL EQAWMNEKFA PELLESKPEI VECVMEQLEH MEENLRRAKR EDLKVSIHQM EMERIRYVLS SYLRCRLMKI EKFFPHVLEK EKTRPEGEPS SLSPEELAFA REFMANTESY LKNVALKHMP PNLQKVDLFR AVPKPDLDSY VFLRVRERQE NILVEPDTDE QRDYVIDLEK GSQHLIRYKT IAPLVASGAV QLI |
GINS4, a member of the GINS4/SLD5 family, has a key part in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is significant for GINS complex assembly. GINS complex connects favorably to singlestranded DNA. Recombinant human GINS4 protein, fused to His-tag at N-terminus was purified by using conventional chromatography techniques.
GINS4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 243 amino acids (1-223 a.a.) and having a molecular mass of 28.2kDa.GINS4 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GINS4: The GINS complex plays an essential role in the initiation of DNA replication, and progression of DNA replication forks. GINS4 is important for GINS complex assembly. GINS complex seems to bind preferentially to single-stranded DNA. Belongs to the GINS4/SLD5 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 8p11.21 Cellular Component: nucleoplasm; cytoplasm Molecular Function:protein binding Biological Process: mitotic cell cycle; DNA strand elongation during DNA replication; inner cell mass cell proliferation |
| NCBI Summary: | The yeast heterotetrameric GINS complex is made up of Sld5, Psf1 (GINS1; MIM 610608), Psf2 (GINS2; MIM 610609), and Psf3 (GINS3; MIM 610610). The formation of the GINS complex is essential for the initiation of DNA replication in yeast and Xenopus egg extracts (Ueno et al., 2005 [PubMed 16287864]). See GINS1 for additional information about the GINS complex.[supplied by OMIM, Mar 2008] |
| UniProt Code: | Q9BRT9 |
| NCBI GenInfo Identifier: | 14150122 |
| NCBI Gene ID: | 84296 |
| NCBI Accession: | NP_115712.1 |
| UniProt Secondary Accession: | Q9BRT9,Q99LZ3, Q499W2, |
| UniProt Related Accession: | Q9BRT9 |
| Molecular Weight: | 11,844 Da |
| NCBI Full Name: | DNA replication complex GINS protein SLD5 |
| NCBI Synonym Full Names: | GINS complex subunit 4 (Sld5 homolog) |
| NCBI Official Symbol: | GINS4�� |
| NCBI Official Synonym Symbols: | SLD5�� |
| NCBI Protein Information: | DNA replication complex GINS protein SLD5; SLD5 homolog |
| UniProt Protein Name: | DNA replication complex GINS protein SLD5 |
| UniProt Synonym Protein Names: | GINS complex subunit 4DNA replication complex GINS protein SLD5, N-terminally processed |
| Protein Family: | DNA replication complex GINS protein |
| UniProt Gene Name: | GINS4�� |
| UniProt Entry Name: | SLD5_HUMAN |
