The Human Gastric Intrinsic Factor CLIA Kit is a cutting-edge diagnostic tool designed for the precise measurement of gastric intrinsic factor levels in human samples, including serum, plasma, and cell culture supernatants. This kit is well-known for its exceptional sensitivity and specificity, ensuring accurate and reproducible results that are crucial for various research applications.Gastric intrinsic factor is a vital protein that plays a key role in the absorption of vitamin B12 in the stomach. Deficiencies in this factor can lead to pernicious anemia and other health issues related to B12 deficiency.
Therefore, the Human Gastric Intrinsic Factor CLIA Kit is a valuable resource for studying and understanding these conditions, as well as for developing potential diagnostic and therapeutic strategies.Overall, this innovative kit offers researchers a reliable and efficient way to analyze gastric intrinsic factor levels, advancing the field of gastrointestinal research and improving patient care. Visit the provided URL for more information and to purchase your kit today.
Product Name:
Human GIF (Gastric Intrinsic Factor) CLIA Kit
SKU:
HUES00281
Target:
Human GIF (Gastric Intrinsic Factor)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GIF. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GIF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GIF, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GIF. You can calculate the concentration of Human GIF in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
95-107
98-113
87-100
Average (%)
100
105
93
1:4
Range (%)
89-102
101-119
97-113
Average (%)
94
109
103
1:8
Range (%)
95-109
98-114
97-112
Average (%)
103
104
105
1:16
Range (%)
98-113
102-116
102-115
Average (%)
105
110
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
86-99
92
EDTA plasma (n=5)
95-112
102
Cell culture media (n=5)
100-114
108
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.83
6.37
16.25
1.93
6.42
16.73
Standard deviation
0.18
0.45
1.58
0.2
0.65
1.6
C V (%)
9.84
7.06
9.72
10.36
10.12
9.56
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GIF concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GIF in samples. No significant cross-reactivity or interference between Human GIF and analogues was observed.
