The Human Growth Hormone CLIA Kit is a cutting-edge tool for the precise measurement of growth hormone levels in human samples such as serum, plasma, and cell culture supernatants. With its exceptional sensitivity and specificity, this kit delivers accurate and dependable results, making it the perfect choice for various research applications.Growth hormone, also known as somatotropin, plays a crucial role in stimulating growth, cell regeneration, and metabolism in the human body.
It is vital for normal growth and development, making it a key biomarker in studies related to growth disorders, obesity, and metabolic diseases.With the Human Growth Hormone CLIA Kit, researchers can gain valuable insights into the regulation and function of growth hormone, paving the way for advancements in the understanding and treatment of growth-related conditions.
Product Name:
Human GH (Growth Hormone) CLIA Kit
SKU:
HUES00151
Target:
Human GH (Growth Hormone)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GH. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GH, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human GH. You can calculate the concentration of Human GH in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-97
104-117
87-102
Average (%)
91
110
93
1:4
Range (%)
90-104
95-110
88-102
Average (%)
95
102
94
1:8
Range (%)
87-100
86-98
86-98
Average (%)
92
93
91
1:16
Range (%)
104-116
88-100
97-111
Average (%)
110
94
103
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
98-113
104
EDTA plasma (n=5)
87-103
94
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
246.6
702.2
1951.13
230.55
767.96
1858.66
Standard deviation
27.45
73.87
168.38
25.25
66.97
200.74
C V (%)
11.13
10.52
8.63
10.95
8.72
10.8
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human GH concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human GH in samples. No significant cross-reactivity or interference between Human GH and analogues was observed.
