Human GGH Recombinant Protein (RPPB1688)
- SKU:
- RPPB1688
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q92820
- Research Area:
- Enzymes
Description
| Product Name: | Human GGH Recombinant Protein |
| Product Code: | RPPB1688 |
| Size: | 20µg |
| Species: | Human |
| Target: | GGH |
| Synonyms: | Gamma-glutamyl hydrolase (conjugase, folylpolygammaglutamyl hydrolase), Gamma-Glu-X carboxypeptidase, gamma-glutamyl hydrolase, Conjugase, GH, EC 3.4.19.9. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The GGH solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MRPHGDTAKK PIIGILMQKC RNKVMKNYGR YYIAASYVKY LESAGARVVP VRLDLTEKDY EILFKSINGI LFPGGSVDLR RSDYAKVAKI FYNLSIQSFD DGDYFPVWGT CLGFEELSLL ISGECLLTAT DTVDVAMPLN FTGGQLHSRM FQNFPTELLL SLAVEPLTAN FHKWSLSVKN FTMNEKLKKF FNVLTTNTDG KIEFISTMEG YKYPVYGVQW HPEKAPYEWK NLDGISHAPN AVKTAFYLAE FFVNEARKNN HHFKSESEEE KALIYQFSPI YTGNISSFQQ CYIFD |
GGH is a homodimeric protein which catalyzes the cleavage of the gamma-glutamyl chain of folylpoly-gamma-glutamyl substrates. GGH is a vital enzyme in folyl and antifolyl poly-gamma-glutamate metabolism and it has a significant part in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of antifolates and pteroylpolyglutamates.
GGH Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 315 amino acids (25-318) and having a molecular mass of 35.9kDa.GGH is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | GGH: Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha- glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Belongs to the peptidase C26 family. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Cofactor and Vitamin Metabolism - folate biosynthesis; Secreted; Protease; EC 3.4.19.9 Chromosomal Location of Human Ortholog: 8q12.3 Cellular Component: extracellular space; nucleus; vacuole Molecular Function:exopeptidase activity; omega peptidase activity Biological Process: tetrahydrofolylpolyglutamate metabolic process |
| NCBI Summary: | This gene catalyzes the hydrolysis of folylpoly-gamma-glutamates and antifolylpoly-gamma-glutamates by the removal of gamma-linked polyglutamates and glutamate. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q92820 |
| NCBI GenInfo Identifier: | 6016127 |
| NCBI Gene ID: | 8836 |
| NCBI Accession: | Q92820.2 |
| UniProt Related Accession: | Q92820 |
| Molecular Weight: | 35,964 Da |
| NCBI Full Name: | Gamma-glutamyl hydrolase |
| NCBI Synonym Full Names: | gamma-glutamyl hydrolase |
| NCBI Official Symbol: | GGH�� |
| NCBI Official Synonym Symbols: | GH�� |
| NCBI Protein Information: | gamma-glutamyl hydrolase |
| UniProt Protein Name: | Gamma-glutamyl hydrolase |
| UniProt Synonym Protein Names: | Conjugase; GH; Gamma-Glu-X carboxypeptidase |
| Protein Family: | Gamma-glutamyl hydrolase |
| UniProt Gene Name: | GGH�� |
| UniProt Entry Name: | GGH_HUMAN |
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